BRENDA - Enzyme Database

The c-Myc target gene Rcl (C6orf108) encodes a novel enzyme, deoxynucleoside 5-monophosphate N-glycosidase

Ghiorghi, Y.K.; Zeller, K.I.; Dang, C.V.; Kaminski, P.A.; J. Biol. Chem. 282, 8150-8156 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.2.6
gene Rc1, expression of soluble unmodified enzyme and His-tagged enzymes in Escherichia coli strain Bli5 (BL21 (DE3) pDIA17)
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.6
E105A
site-directed mutagenesis, the mutant shows about 200fold reduced activity and a 170fold increased Km compared to the wild-type enzyme
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
6-methylthio-GMP
competitive versus dGMP
Rattus norvegicus
2.4.2.6
GMP
competitive versus dGMP
Rattus norvegicus
2.4.2.6
Zn2+
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
additional information
-
additional information
kinetics and substrate specifiicty
Rattus norvegicus
2.4.2.6
0.048
-
dGMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.25
-
dAMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.45
-
dIMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
4
-
dCMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
5
-
dGMP
pH 7.5, 37°C, recombinant mutant E105A
Rattus norvegicus
2.4.2.6
15
-
dUMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
50
-
dTMP
above, pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.6
additional information
no effect by Ca2+, Mg2+, Na+, and K+
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
17649
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE; 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
19812
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
20440
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
Rattus norvegicus
2.4.2.6
40520
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Rattus norvegicus
-
gene Rc1
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.6
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
2.4.2.6
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
Glu105, Asp-99 and Asn-172 are catalytic active site residues
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.6
fibroblast
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.6
-20°C, purified recombinant enzyme, 1 mg/ml, at least 1 year without loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
dAMP + base
-
674900
Rattus norvegicus
dNMP + adenine
-
-
-
r
2.4.2.6
dCMP + base
-
674900
Rattus norvegicus
dNMP + cytidine
-
-
-
r
2.4.2.6
dGMP + base
dGMP is the best substrate
674900
Rattus norvegicus
dNMP + guanine
-
-
-
r
2.4.2.6
dIMP + base
-
674900
Rattus norvegicus
dNMP + hypoxanthine
-
-
-
r
2.4.2.6
dTMP + base
-
674900
Rattus norvegicus
dNMP + thymine
-
-
-
r
2.4.2.6
dUMP + base
-
674900
Rattus norvegicus
dNMP + uracil
-
-
-
r
2.4.2.6
additional information
RCL cleaves the N-glycosidic bond of deoxynucleoside 5'-monophosphates to yield two reaction products, deoxyribose 5-phosphate and purine or pyrimidine base
674900
Rattus norvegicus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.4.2.6
dimer
2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
monomer
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
More
at 0.003 mM the His-RCL is mostly monomeric, at 0.03 mM, His-RCL is mostly dimeric, at 0.01 mM His-RCL is at equilibrium between monomeric and dimeric states, overview
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.6
37
-
assay at
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.0066
-
dAMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.0066
-
dIMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.0178
-
dGMP
pH 7.5, 37°C, recombinant mutant E105A
Rattus norvegicus
2.4.2.6
0.0231
-
dCMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.0297
-
dGMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.158
-
dUMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
6
-
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.6
5
8
50% of maximal activity at pH 7.0 with substrate dCMP, 20% with dGMP, 10% of maximal activity at pH 8.0 with both substrates
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.01
-
6-methylthio-GMP
pH 7.5, 37°C, recombinant wild-type enzyme, versus dGMP
Rattus norvegicus
2.4.2.6
0.02
-
GMP
pH 7.5, 37°C, recombinant wild-type enzyme, versus dGMP
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
gene Rc1, expression of soluble unmodified enzyme and His-tagged enzymes in Escherichia coli strain Bli5 (BL21 (DE3) pDIA17)
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.6
E105A
site-directed mutagenesis, the mutant shows about 200fold reduced activity and a 170fold increased Km compared to the wild-type enzyme
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
6-methylthio-GMP
competitive versus dGMP
Rattus norvegicus
2.4.2.6
GMP
competitive versus dGMP
Rattus norvegicus
2.4.2.6
Zn2+
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.01
-
6-methylthio-GMP
pH 7.5, 37°C, recombinant wild-type enzyme, versus dGMP
Rattus norvegicus
2.4.2.6
0.02
-
GMP
pH 7.5, 37°C, recombinant wild-type enzyme, versus dGMP
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
additional information
-
additional information
kinetics and substrate specifiicty
Rattus norvegicus
2.4.2.6
0.048
-
dGMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.25
-
dAMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.45
-
dIMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
4
-
dCMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
5
-
dGMP
pH 7.5, 37°C, recombinant mutant E105A
Rattus norvegicus
2.4.2.6
15
-
dUMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
50
-
dTMP
above, pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.6
additional information
no effect by Ca2+, Mg2+, Na+, and K+
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
17649
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE; 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
19812
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
20440
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
Rattus norvegicus
2.4.2.6
40520
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.6
fibroblast
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.6
-20°C, purified recombinant enzyme, 1 mg/ml, at least 1 year without loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
dAMP + base
-
674900
Rattus norvegicus
dNMP + adenine
-
-
-
r
2.4.2.6
dCMP + base
-
674900
Rattus norvegicus
dNMP + cytidine
-
-
-
r
2.4.2.6
dGMP + base
dGMP is the best substrate
674900
Rattus norvegicus
dNMP + guanine
-
-
-
r
2.4.2.6
dIMP + base
-
674900
Rattus norvegicus
dNMP + hypoxanthine
-
-
-
r
2.4.2.6
dTMP + base
-
674900
Rattus norvegicus
dNMP + thymine
-
-
-
r
2.4.2.6
dUMP + base
-
674900
Rattus norvegicus
dNMP + uracil
-
-
-
r
2.4.2.6
additional information
RCL cleaves the N-glycosidic bond of deoxynucleoside 5'-monophosphates to yield two reaction products, deoxyribose 5-phosphate and purine or pyrimidine base
674900
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.2.6
dimer
2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
monomer
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
Rattus norvegicus
2.4.2.6
More
at 0.003 mM the His-RCL is mostly monomeric, at 0.03 mM, His-RCL is mostly dimeric, at 0.01 mM His-RCL is at equilibrium between monomeric and dimeric states, overview
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.6
37
-
assay at
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.0066
-
dAMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.0066
-
dIMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.0178
-
dGMP
pH 7.5, 37°C, recombinant mutant E105A
Rattus norvegicus
2.4.2.6
0.0231
-
dCMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.0297
-
dGMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
2.4.2.6
0.158
-
dUMP
pH 7.5, 37°C, recombinant wild-type enzyme
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
6
-
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.6
5
8
50% of maximal activity at pH 7.0 with substrate dCMP, 20% with dGMP, 10% of maximal activity at pH 8.0 with both substrates
Rattus norvegicus