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Literature summary extracted from

  • Praetorius-Ibba, M.; Hausmann, C.D.; Paras, M.; Rogers, T.E.; Ibba, M.
    Functional association between three archaeal aminoacyl-tRNA synthetases (2007), J. Biol. Chem., 282, 3680-3687.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.4 additional information a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview Methanothermobacter thermautotrophicus
6.1.1.15 additional information a complex between ProRS and leucyl-tRNA synthetase, LeuRS, in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview Methanothermobacter thermautotrophicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expression of prolyl-tRNA synthetase, ProRS, and LeuRS in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged LeuRS in Escherichia coli strain BL21(DE3) Methanothermobacter thermautotrophicus
6.1.1.6 expressed in Escherichia coli Methanothermobacter thermautotrophicus
6.1.1.15 expression of ProRS and leucyl-tRNA synthetase, LeuRS, in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged ProRS in Escherichia coli strain BL21(DE3) Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 additional information
-
additional information prolyl-tRNA synthetase, ProRS, and LeuRS interaction kinetics Methanothermobacter thermautotrophicus
6.1.1.6 0.00074
-
tRNALys at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin Methanothermobacter thermautotrophicus
6.1.1.6 0.0066
-
L-lysine at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin Methanothermobacter thermautotrophicus
6.1.1.15 additional information
-
additional information ProRS and leucyl-tRNA synthetase, LeuRS, interaction kinetics Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 Mg2+
-
Methanothermobacter thermautotrophicus
6.1.1.15 Mg2+
-
Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Methanothermobacter thermautotrophicus a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.15 ATP + L-proline + tRNAPro Methanothermobacter thermautotrophicus a complex between ProRS and leucyl-tRNA synthetase, LeuRS, in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Methanothermobacter thermautotrophicus
-
-
-
6.1.1.6 Methanothermobacter thermautotrophicus
-
-
-
6.1.1.15 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant His6-tagged LeuRS from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and ultrafiltration, co-purification of prolyl-tRNA synthetase, ProRS, and LeuRS, overview Methanothermobacter thermautotrophicus
6.1.1.6 Sephacryl S300 26/60 column chromatography and HiPrep 16/10 Q Sepharose FF column chromatography Methanothermobacter thermautotrophicus
6.1.1.15 recombinant His6-tagged ProRS from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and ultrafiltration, co-purification of ProRS and leucyl-tRNA synthetase, LeuRS, overview Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
Methanothermobacter thermautotrophicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview Methanothermobacter thermautotrophicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.6 ATP + L-lysine + tRNALys
-
Methanothermobacter thermautotrophicus AMP + L-lysyl-tRNALys + diphosphate
-
?
6.1.1.15 ATP + L-proline + tRNAPro
-
Methanothermobacter thermautotrophicus AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro a complex between ProRS and leucyl-tRNA synthetase, LeuRS, in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview Methanothermobacter thermautotrophicus AMP + diphosphate + L-prolyl-tRNAPro
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucyl-tRNA synthetase
-
Methanothermobacter thermautotrophicus
6.1.1.4 LeuRS
-
Methanothermobacter thermautotrophicus
6.1.1.6 LysRS1
-
Methanothermobacter thermautotrophicus
6.1.1.6 Lysyl-tRNA synthetase
-
Methanothermobacter thermautotrophicus
6.1.1.15 Prolyl-tRNA synthetase
-
Methanothermobacter thermautotrophicus
6.1.1.15 ProRS
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 22
-
assay at room temperature Methanothermobacter thermautotrophicus
6.1.1.15 22
-
assay at room temperature Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.6 0.0143
-
L-lysine at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 7.5
-
assay at Methanothermobacter thermautotrophicus
6.1.1.15 7.5
-
assay at Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
Methanothermobacter thermautotrophicus
6.1.1.6 ATP
-
Methanothermobacter thermautotrophicus
6.1.1.15 ATP
-
Methanothermobacter thermautotrophicus