EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | additional information | a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | Methanothermobacter thermautotrophicus | |
6.1.1.15 | additional information | a complex between ProRS and leucyl-tRNA synthetase, LeuRS, in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | Methanothermobacter thermautotrophicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.4 | expression of prolyl-tRNA synthetase, ProRS, and LeuRS in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged LeuRS in Escherichia coli strain BL21(DE3) | Methanothermobacter thermautotrophicus |
6.1.1.6 | expressed in Escherichia coli | Methanothermobacter thermautotrophicus |
6.1.1.15 | expression of ProRS and leucyl-tRNA synthetase, LeuRS, in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged ProRS in Escherichia coli strain BL21(DE3) | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.4 | additional information | - |
additional information | prolyl-tRNA synthetase, ProRS, and LeuRS interaction kinetics | Methanothermobacter thermautotrophicus | |
6.1.1.6 | 0.00074 | - |
tRNALys | at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin | Methanothermobacter thermautotrophicus | |
6.1.1.6 | 0.0066 | - |
L-lysine | at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin | Methanothermobacter thermautotrophicus | |
6.1.1.15 | additional information | - |
additional information | ProRS and leucyl-tRNA synthetase, LeuRS, interaction kinetics | Methanothermobacter thermautotrophicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | Mg2+ | - |
Methanothermobacter thermautotrophicus | |
6.1.1.15 | Mg2+ | - |
Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | Methanothermobacter thermautotrophicus | a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | Methanothermobacter thermautotrophicus | a complex between ProRS and leucyl-tRNA synthetase, LeuRS, in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.4 | Methanothermobacter thermautotrophicus | - |
- |
- |
6.1.1.6 | Methanothermobacter thermautotrophicus | - |
- |
- |
6.1.1.15 | Methanothermobacter thermautotrophicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.4 | recombinant His6-tagged LeuRS from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and ultrafiltration, co-purification of prolyl-tRNA synthetase, ProRS, and LeuRS, overview | Methanothermobacter thermautotrophicus |
6.1.1.6 | Sephacryl S300 26/60 column chromatography and HiPrep 16/10 Q Sepharose FF column chromatography | Methanothermobacter thermautotrophicus |
6.1.1.15 | recombinant His6-tagged ProRS from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and ultrafiltration, co-purification of ProRS and leucyl-tRNA synthetase, LeuRS, overview | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.4 | ATP + L-leucine + tRNALeu | a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.6 | ATP + L-lysine + tRNALys | - |
Methanothermobacter thermautotrophicus | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | a complex between ProRS and leucyl-tRNA synthetase, LeuRS, in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.4 | Leucyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.4 | LeuRS | - |
Methanothermobacter thermautotrophicus |
6.1.1.6 | LysRS1 | - |
Methanothermobacter thermautotrophicus |
6.1.1.6 | Lysyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.15 | Prolyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.15 | ProRS | - |
Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 22 | - |
assay at room temperature | Methanothermobacter thermautotrophicus |
6.1.1.15 | 22 | - |
assay at room temperature | Methanothermobacter thermautotrophicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.0143 | - |
L-lysine | at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 7.5 | - |
assay at | Methanothermobacter thermautotrophicus |
6.1.1.15 | 7.5 | - |
assay at | Methanothermobacter thermautotrophicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | ATP | - |
Methanothermobacter thermautotrophicus | |
6.1.1.6 | ATP | - |
Methanothermobacter thermautotrophicus | |
6.1.1.15 | ATP | - |
Methanothermobacter thermautotrophicus |