EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.5 | expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.5 | 0.005 | - |
ATP | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 0.0077 | - |
ADP | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 0.06 | - |
CoA | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 1.4 | - |
phosphate | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 2.8 | - |
succinate | at 55°C | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.5 | 27000 | - |
SDS-PAGE, beta subunit | Thermococcus kodakarensis |
6.2.1.5 | 50000 | - |
SDS-PAGE, alpha subunit | Thermococcus kodakarensis |
6.2.1.5 | 160000 | - |
circa 160000 Da, gel filtration | Thermococcus kodakarensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.5 | Thermococcus kodakarensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.5 | Resource Q column chromatography, Resource ISO column chromatography, and Superdex 200 HR 10/30 gel filtration | Thermococcus kodakarensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.5 | 0.18 | - |
cell extract | Thermococcus kodakarensis |
6.2.1.5 | 39.4 | - |
after 221fold purification | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.5 | ATP + adipate + CoA | 59% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + adipyl-CoA | - |
r | |
6.2.1.5 | ATP + butyrate + CoA | 48% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + butyryl-CoA | - |
r | |
6.2.1.5 | ATP + glutarate + CoA | 121% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + glutaryl-CoA | - |
r | |
6.2.1.5 | ATP + oxalate + CoA | 9% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + oxalyl-CoA | - |
r | |
6.2.1.5 | ATP + propionate + CoA | 10% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + propionyl-CoA | - |
r | |
6.2.1.5 | ATP + succinate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + succinyl-CoA | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.5 | heterotetramer | 2 * 50000 + 2 * 27000, gel filtration | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.5 | ADP-forming succinyl-CoA synthetase | - |
Thermococcus kodakarensis |
6.2.1.5 | SCS | - |
Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.5 | 75 | 80 | - |
Thermococcus kodakarensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.5 | 20 | - |
phosphate | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 22 | - |
ADP | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 23 | - |
ATP | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 25 | - |
CoA | at 55°C | Thermococcus kodakarensis | |
6.2.1.5 | 29 | - |
succinate | at 55°C | Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.5 | 6.5 | - |
- |
Thermococcus kodakarensis |