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Literature summary extracted from

  • Shikata, K.; Fukui, T.; Atomi, H.; Imanaka, T.
    A novel ADP-forming succinyl-CoA synthetase in Thermococcus kodakaraensis structurally related to the archaeal nucleoside diphosphate-forming acetyl-CoA synthetases (2007), J. Biol. Chem., 282, 26963-26970.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.5 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.5 0.005
-
ATP at 55°C Thermococcus kodakarensis
6.2.1.5 0.0077
-
ADP at 55°C Thermococcus kodakarensis
6.2.1.5 0.06
-
CoA at 55°C Thermococcus kodakarensis
6.2.1.5 1.4
-
phosphate at 55°C Thermococcus kodakarensis
6.2.1.5 2.8
-
succinate at 55°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.5 27000
-
SDS-PAGE, beta subunit Thermococcus kodakarensis
6.2.1.5 50000
-
SDS-PAGE, alpha subunit Thermococcus kodakarensis
6.2.1.5 160000
-
circa 160000 Da, gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.5 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.5 Resource Q column chromatography, Resource ISO column chromatography, and Superdex 200 HR 10/30 gel filtration Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.5 0.18
-
cell extract Thermococcus kodakarensis
6.2.1.5 39.4
-
after 221fold purification Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.5 ATP + adipate + CoA 59% activity compared to succinate Thermococcus kodakarensis ADP + phosphate + adipyl-CoA
-
r
6.2.1.5 ATP + butyrate + CoA 48% activity compared to succinate Thermococcus kodakarensis ADP + phosphate + butyryl-CoA
-
r
6.2.1.5 ATP + glutarate + CoA 121% activity compared to succinate Thermococcus kodakarensis ADP + phosphate + glutaryl-CoA
-
r
6.2.1.5 ATP + oxalate + CoA 9% activity compared to succinate Thermococcus kodakarensis ADP + phosphate + oxalyl-CoA
-
r
6.2.1.5 ATP + propionate + CoA 10% activity compared to succinate Thermococcus kodakarensis ADP + phosphate + propionyl-CoA
-
r
6.2.1.5 ATP + succinate + CoA
-
Thermococcus kodakarensis ADP + phosphate + succinyl-CoA
-
r

Subunits

EC Number Subunits Comment Organism
6.2.1.5 heterotetramer 2 * 50000 + 2 * 27000, gel filtration Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
6.2.1.5 ADP-forming succinyl-CoA synthetase
-
Thermococcus kodakarensis
6.2.1.5 SCS
-
Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.5 75 80
-
Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.5 20
-
phosphate at 55°C Thermococcus kodakarensis
6.2.1.5 22
-
ADP at 55°C Thermococcus kodakarensis
6.2.1.5 23
-
ATP at 55°C Thermococcus kodakarensis
6.2.1.5 25
-
CoA at 55°C Thermococcus kodakarensis
6.2.1.5 29
-
succinate at 55°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.5 6.5
-
-
Thermococcus kodakarensis