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Literature summary extracted from

  • Jin, M.; Yuan, Q.; Li, S.; Travis, G.H.
    Role of LRAT on the retinoid isomerase activity and membrane association of Rpe65 (2007), J. Biol. Chem., 282, 20915-20924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 stable expression in HEK-293T cells Mus musculus
2.3.1.135 stable expression in HEK-293T cells Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.135 Ca2+
-
 Mus musculus
2.3.1.135 Ca2+
-
 Bos taurus
2.3.1.135 Mg2+
-
 Mus musculus
2.3.1.135 Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.135 lecithin + retinol-[cellular retinol-binding protein] Mus musculus
-
 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein]
-
 ?
2.3.1.135 lecithin + retinol-[cellular retinol-binding protein] Bos taurus
-
 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein]
-
 ?
2.3.1.135 lecithin + retinol-[cellular retinol-binding protein] Mus musculus 129S2/Sv
-
 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein]
-
 ?
2.3.1.135 additional information Mus musculus LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview ?
-
 ?
2.3.1.135 additional information Bos taurus LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview ?
-
 ?
2.3.1.135 additional information Mus musculus 129S2/Sv LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Bos taurus
-
-
-
2.3.1.135 Mus musculus
-
-
-
2.3.1.135 Mus musculus 129S2/Sv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 native enzyme partially by membrane preparation from retinal pigment epithelium Mus musculus
2.3.1.135 native enzyme partially by membrane preparation from retinal pigment epithelium Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.135 eye
-
 Mus musculus
-
2.3.1.135 eye
-
 Bos taurus
-
2.3.1.135 retinal pigment epithelium
-
 Mus musculus
-
2.3.1.135 retinal pigment epithelium
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 lecithin + retinol-[cellular retinol-binding protein]
-
 Mus musculus 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein]
-
 ?
2.3.1.135 lecithin + retinol-[cellular retinol-binding protein]
-
 Bos taurus 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein]
-
 ?
2.3.1.135 lecithin + retinol-[cellular retinol-binding protein]
-
 Mus musculus 129S2/Sv 2-acylglycerophosphocholine + retinyl ester-[cellular retinol-binding protein]
-
 ?
2.3.1.135 additional information LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview Mus musculus ?
-
 ?
2.3.1.135 additional information LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview Bos taurus ?
-
 ?
2.3.1.135 additional information no activity with Rpe65 protein Mus musculus ?
-
 ?
2.3.1.135 additional information no activity with Rpe65 protein Bos taurus ?
-
 ?
2.3.1.135 additional information LRAT is not required for retinoid isomerase activity beyond synthesis of retinyl-ester substrate, association of Rpe65 protein, a membrane-associated protein in the retinal pigment epithelium, with membranes is neither dependent upon LRAT nor the result of S-palmitoylation on Cys231, Cys329, and Cys330 by the enzyme, overview Mus musculus 129S2/Sv ?
-
 ?
2.3.1.135 additional information no activity with Rpe65 protein Mus musculus 129S2/Sv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin:retinol acyl transferase
-
 Mus musculus
2.3.1.135 lecithin:retinol acyl transferase
-
 Bos taurus
2.3.1.135 LRAT
-
 Mus musculus
2.3.1.135 LRAT
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.135 37
-
 assay at Mus musculus
2.3.1.135 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.135 7.5
-
 assay at Mus musculus
2.3.1.135 7.5
-
 assay at Bos taurus