EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.8 | NH4Cl | maximal stimulation at 40 mM, 3fold | Salmonella enterica | |
2.3.1.8 | NH4Cl | 3fold stimulation at 40 mM. No significant increase in activity above 40 mM | Salmonella enterica | |
2.3.1.8 | pyruvate | stimulates activity of wild-type enzyme 0.2fold over control, and the activity of the mutant enzyme R252H 3fold over control | Salmonella enterica | |
2.3.1.8 | pyruvate | stimulates wild-type activity, its effect is potentiated in the variants, being most pronounced on R252H | Salmonella enterica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.8 | expression in Escherichia coli | Salmonella enterica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.8 | G273D | kcat for reaction with acetyl-CoA and phosphate is 3fold higher than wild-type value, kcat for reaction with CoA and acetyl phosphate is 2.3fold higher than wild-type value. Mutant enzyme shows less aggregation than wild type enzyme | Salmonella enterica |
2.3.1.8 | G273D | kcat/Km for CoA is 2.2fold higher than wild-type value. kcat/KM for acetoacetyl-CoA is 3.6fold higher than wild-type value. Lower proportion of large enzyme aggregates compared with wild-type enzyme | Salmonella enterica |
2.3.1.8 | M294I | kcat for reaction with acetyl-CoA and phosphate is 143fold lower than wild-type value, kcat for reaction with CoA and acetyl phosphate is 1.4fold lower than wild-type value. Mutant enzyme shows less aggregation than wild type enzyme | Salmonella enterica |
2.3.1.8 | M294I | kcat/Km for CoA is 1.7fold higher than wild-type value. kcat/KM for acetoacetyl-CoA is 1.2lower higher than wild-type value. Lower proportion of large enzyme aggregates compared with wild-type enzyme | Salmonella enterica |
2.3.1.8 | R252H | kcat for reaction with acetyl-CoA and phosphate is 2.5fold higher than wild-type value, kcat for reaction with CoA and acetyl phosphate is 2.5fold higher than wild-type value. No inhibition by NADH. Mutant enzyme shows less aggregation than wild type enzyme | Salmonella enterica |
2.3.1.8 | R252H | kcat/Km for CoA is 2.6fold higher than wild-type value. kcat/KM for acetoacetyl-CoA is 1.6fold higher than wild-type value. Lower proportion of large enzyme aggregates compared with wild-type enzyme | Salmonella enterica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.8 | NADH | inhibits by changing enzyme conformation, pyruvate counteracts the inhibitory effect of NADH; inhibits by changing the conformation of the enzyme | Salmonella enterica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.8 | 0.1621 | - |
CoA | wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 0.1621 | - |
CoA | 37°C, pH 7.5, wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 0.163 | - |
CoA | 37°C, pH 7.5, mutant enzyme R252H | Salmonella enterica | |
2.3.1.8 | 0.163 | - |
CoA | mutant enzyme R252H | Salmonella enterica | |
2.3.1.8 | 0.168 | - |
CoA | 37°C, pH 7.5, mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 0.1683 | - |
CoA | mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 0.192 | - |
CoA | 37°C, pH 7.5, mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 0.192 | - |
CoA | mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 0.2812 | - |
acetyl-CoA | 37°C, pH 7.5, mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 0.2812 | - |
acetyl-CoA | mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 0.2814 | - |
acetyl-CoA | 37°C, pH 7.5, mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 0.2814 | - |
acetyl-CoA | mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 0.3293 | - |
acetyl-CoA | wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 0.3293 | - |
acetyl-CoA | 37°C, pH 7.5, wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 0.5297 | - |
acetyl-CoA | 37°C, pH 7.5, mutant enzyme R252H | Salmonella enterica | |
2.3.1.8 | 0.5297 | - |
acetyl-CoA | mutant enzyme R252H | Salmonella enterica | |
2.3.1.8 | 1.1 | - |
phosphate | 37°C, pH 7.5, mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 1.1 | - |
phosphate | mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 1.3 | - |
phosphate | 37°C, pH 7.5, mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 1.3 | - |
phosphate | mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 1.5 | - |
phosphate | wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 1.5 | - |
phosphate | 37°C, pH 7.5, wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 2.8 | - |
phosphate | 37°C, pH 7.5, mutant enzyme R252H | Salmonella enterica | |
2.3.1.8 | 2.8 | - |
phosphate | mutant enzyme R252H | Salmonella enterica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.8 | KCl | stimulates | Salmonella enterica | |
2.3.1.8 | KCl | stimulates enzyme activity 2.5-fold | Salmonella enterica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 490000 | - |
gel filtration | Salmonella enterica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.8 | Salmonella enterica | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.8 | acetyl-CoA + phosphate | - |
Salmonella enterica | CoA + acetyl phosphate | - |
? | |
2.3.1.8 | CoA + acetyl phosphate | - |
Salmonella enterica | acetyl-CoA + phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.8 | hexamer | it is possible that native Pta is a dimer of trimers | Salmonella enterica |
2.3.1.8 | trimer | wild-type enzyme is a trimer. Pta variants formmore hexamer than the wild-typ protein | Salmonella enterica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.8 | 1.73 | - |
acetyl-CoA | 37°C, pH 7.5, wild-type enzyme, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 25 | - |
acetyl-CoA | 37°C, pH 7.5, mutant enzyme R252H, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 57.9 | - |
acetyl-CoA | 37°C, pH 7.5, mutant enzyme M294I, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 57.9 | - |
acetyl-CoA | mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 83.1 | - |
acetyl-CoA | wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 208.9 | - |
acetyl-CoA | mutant enzyme R252H | Salmonella enterica | |
2.3.1.8 | 252.5 | - |
acetyl-CoA | 37°C, pH 7.5, mutant enzyme G273D, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 252.5 | - |
acetyl-CoA | mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 403 | - |
CoA | 37°C, pH 7.5, mutant enzyme M294I, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 403.5 | - |
acetyl phosphate | mutant enzyme M294I | Salmonella enterica | |
2.3.1.8 | 574.5 | - |
acetyl phosphate | wild-type enzyme | Salmonella enterica | |
2.3.1.8 | 574.5 | - |
CoA | 37°C, pH 7.5, wild-type enzyme, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 1301 | - |
CoA | 37°C, pH 7.5, mutant enzyme G273D, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 1301 | - |
acetyl phosphate | mutant enzyme G273D | Salmonella enterica | |
2.3.1.8 | 1480 | - |
CoA | 37°C, pH 7.5, mutant enzyme R252H, per trimer of His-tagged enzyme | Salmonella enterica | |
2.3.1.8 | 1480 | - |
acetyl phosphate | mutant enzyme R252H | Salmonella enterica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 7.5 | - |
- |
Salmonella enterica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 7 | 8.5 | pH 7.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity | Salmonella enterica |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.8 | 1.1 | - |
NADH | wild type enzyme | Salmonella enterica | |
2.3.1.8 | 1.1 | - |
NADH | 37°C, pH 7.5, wild-type enzyme | Salmonella enterica |