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Literature summary extracted from

  • Campian, J.L.; Gao, X.; Qian, M.; Eaton, J.W.
    Cytochrome C oxidase activity and oxygen tolerance (2007), J. Biol. Chem., 282, 12430-12438.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.9 additional information overexpression of subunit Vb increases COX activity and decreases reactive oxygen species production in wild-type HeLa-20 cells, along with some increase in tolerance to hyperoxia Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.9 COX subunit Vb open reading frame ligated into the pcDNA3.1/V5-His TOPO-TA expression vector and transfected into TOP10 Escherichia coli. Overexpression in HeLa-20 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 siRNA small interfering RNA against Vb selectively lowers COX Vb expression in HeLa-80 cells, increases mitochondrial reactive oxygen species generation, decreases COX activity 60-80%, and diminishes viability under 80% (but not 20%) O2 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.9 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.9 HeLa-80 cell cells proliferate under 80% O2. Under both 20% and 80% O2, mitochondrial reactive oxygen species production is 2fold less in HeLa-80 cells than in wild-type HeLa cells, related to a 2fold higher cytochrome-c oxidase activity. Elevated enzyme activity is associated with a 2fold increase in the regulatory subunit Vb. Treatment with siRNA against subunit Vb lowers its expression, increases mitochondrial generation of reactive oxygen species, decreases enzyme activity by 60-80% and diminishes viability under 80% O2 Homo sapiens
-
7.1.1.9 HeLa-80 cell elevated COX activity in the oxygen-tolerant HeLa-80 cells, associated with a more than 2fold increase in the regulatory subunit COX Vb, whereas expression levels of other subunits are very close to wild-type HeLa-20 cells Homo sapiens
-
7.1.1.9 additional information HeLa-20 cell Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 + H+
-
 Homo sapiens ferricytochrome c + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.9 COX Vb
-
 Homo sapiens
7.1.1.9 cytochrome c oxidase
-
 Homo sapiens