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Literature summary extracted from

  • Hasan, Z.; Renirie, R.; Kerkman, R.; Ruijssenaars, H.J.; Hartog, A.F.; Wever, R.
    Laboratory-evolved vanadium chloroperoxidase exhibits 100-fold higher halogenating activity at alkaline pH: catalytic effects from first and second coordination sphere mutations (2006), J. Biol. Chem., 281, 9738-9744.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.B2 expression in Escherichia coli Curvularia inaequalis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.B2 P395D 10fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395D/L241V/T343A 100fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395D/L241V/T343A 100fold increase in brominating activity at pH 8, the brominating activity at pH 5 was increased by a factor of 6 Curvularia inaequalis
1.11.1.B2 P395D/L241V/T343A 40fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395E 10fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395H/A399S 5fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395T 6fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395T/L241V 19fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395T/L241V/T343A 20fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 P395T/T343A 14fold increase in brominating activity at pH 8 Curvularia inaequalis
1.11.1.B2 R360C/I391V 5fold increase in brominating activity at pH 8 Curvularia inaequalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.B2 Vanadium
-
 Curvularia inaequalis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B2 Curvularia inaequalis P49053
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B2 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone, brominating activity of chloroperoxidase Curvularia inaequalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.B2 vanadium chloroperoxidase
-
 Curvularia inaequalis
1.11.1.B2 vCPO
-
 Curvularia inaequalis