BRENDA - Enzyme Database show

Human arsenic methyltransferase (AS3MT) pharmacogenetics: gene resequencing and functional genomics studies

Wood, T.C.; Salavagionne, O.E.; Mukherjee, B.; Wang, L.; Klumpp, A.F.; Thomae, B.A.; Eckloff, B.W.; Schaid, D.J.; Wieben, E.D.; Weinshilboum, R.M.; J. Biol. Chem. 281, 7364-7373 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
after expression in COS-1 cells and correction for transfection efficiency, the Trp173 allozyme displays 31%, Thr287 350%, Ile306 4.8%, and Thr287/Ile306 6.2% of the activity of the wild type allozyme, with 20, 190, 4.4, and 7.9% of the level of wild-type immunoreactive protein, respectively
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.0031
-
S-adenosyl-L-methionine
37°C, pH 8.0, Trp173 allozyme
Homo sapiens
2.1.1.137
0.0045
-
arsenite
37°C, pH 8.0, Thr287 allozyme
Homo sapiens
2.1.1.137
0.0046
-
S-adenosyl-L-methionine
37°C, pH 8.0, wild-type enzyme
Homo sapiens
2.1.1.137
0.0089
-
arsenite
37°C, pH 8.0, Trp173 allozyme
Homo sapiens
2.1.1.137
0.011
-
S-adenosyl-L-methionine
37°C, pH 8.0, Thr287 allozyme
Homo sapiens
2.1.1.137
0.0118
-
arsenite
37°C, pH 8.0, wild-type enzyme
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Homo sapiens
AS3MT may contribute to variation in arsenic metabolism and, perhaps, arsenic-dependent carcinogenesis in humans
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
674763
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
AS3MT may contribute to variation in arsenic metabolism and, perhaps, arsenic-dependent carcinogenesis in humans
674763
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
after expression in COS-1 cells and correction for transfection efficiency, the Trp173 allozyme displays 31%, Thr287 350%, Ile306 4.8%, and Thr287/Ile306 6.2% of the activity of the wild type allozyme, with 20, 190, 4.4, and 7.9% of the level of wild-type immunoreactive protein, respectively
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.0031
-
S-adenosyl-L-methionine
37°C, pH 8.0, Trp173 allozyme
Homo sapiens
2.1.1.137
0.0045
-
arsenite
37°C, pH 8.0, Thr287 allozyme
Homo sapiens
2.1.1.137
0.0046
-
S-adenosyl-L-methionine
37°C, pH 8.0, wild-type enzyme
Homo sapiens
2.1.1.137
0.0089
-
arsenite
37°C, pH 8.0, Trp173 allozyme
Homo sapiens
2.1.1.137
0.011
-
S-adenosyl-L-methionine
37°C, pH 8.0, Thr287 allozyme
Homo sapiens
2.1.1.137
0.0118
-
arsenite
37°C, pH 8.0, wild-type enzyme
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Homo sapiens
AS3MT may contribute to variation in arsenic metabolism and, perhaps, arsenic-dependent carcinogenesis in humans
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
674763
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
AS3MT may contribute to variation in arsenic metabolism and, perhaps, arsenic-dependent carcinogenesis in humans
674763
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?