Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Opaleye, O.; Rose, R.S.; Whittaker, M.M.; Woo, E.J.; Whittaker, J.W.; Pickersgill, R.W.
    Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase (2006), J. Biol. Chem., 281, 6428-6433.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 Formamide specific activity of the wild type oxalate oxidase is slightly higher when 1 M formamide is added Hordeum vulgare

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.4 the mutant proteins expressed in Picha pastoris Hordeum vulgare

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.3.4 hanging-drop vapor diffusion method at 18 °C using 1 micro l of protein (10-15 mg/ml) plus 1 micro l reservoir drops an 1-ml reservoirs, native crystals grown from 2.3M (NH4)2SO4 and 5% 2-propanol are rhombohedral, crystals of recombinant protein, grown from 10% polyethylene glycol 4000 and 0.1 M NaAc, pH 4.6, are tetragonal, Asn75-> Ala OXO are grown from 20% 2-propanol, 0.1 M NaAc, pH 4.6, 0.2 M CaCl2, can be cryo-cooled directly and are rhombohedral Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
1.2.3.4 N75A reduced activity to 2.4% of the wild type level Hordeum vulgare
1.2.3.4 N85A reduced activity to 0.5% of the wild type level Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 additional information for wild type oxalate oxidase, glycolate does not serve as a substrate and does not significantly inhibit turnover when included in the assay at equimolar (20mM) concentrations of oxalate Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.4 NaCl specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NH4Cl Hordeum vulgare
1.2.3.4 NH4Cl specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NaCl Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Hordeum vulgare P45850 barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4 mutant proteins Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.3.4 0.055
-
asparagine mutant (N85A), thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is higher in the presence of 1 M NH4Cl or 1 M NaCl and slightly lower when 1 M formamide is added Hordeum vulgare
1.2.3.4 0.24
-
asparagine mutant (N75A),thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is slightly higher in the presence of 1 M NH4Cl or 1 M NaCl and does not change when 1 M formamide is added Hordeum vulgare
1.2.3.4 10.26
-
wild type, thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 additional information glycolate does not serve as a substrate Hordeum vulgare ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.3.4 OXO
-
Hordeum vulgare