Literature summary extracted from
Opaleye, O.; Rose, R.S.; Whittaker, M.M.; Woo, E.J.; Whittaker, J.W.; Pickersgill, R.W.
Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase (2006), J. Biol. Chem., 281, 6428-6433.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.2.3.4 |
Formamide |
specific activity of the wild type oxalate oxidase is slightly higher when 1 M formamide is added |
Hordeum vulgare |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.2.3.4 |
the mutant proteins expressed in Picha pastoris |
Hordeum vulgare |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.2.3.4 |
hanging-drop vapor diffusion method at 18 °C using 1 micro l of protein (10-15 mg/ml) plus 1 micro l reservoir drops an 1-ml reservoirs, native crystals grown from 2.3M (NH4)2SO4 and 5% 2-propanol are rhombohedral, crystals of recombinant protein, grown from 10% polyethylene glycol 4000 and 0.1 M NaAc, pH 4.6, are tetragonal, Asn75-> Ala OXO are grown from 20% 2-propanol, 0.1 M NaAc, pH 4.6, 0.2 M CaCl2, can be cryo-cooled directly and are rhombohedral |
Hordeum vulgare |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.2.3.4 |
N75A |
reduced activity to 2.4% of the wild type level |
Hordeum vulgare |
1.2.3.4 |
N85A |
reduced activity to 0.5% of the wild type level |
Hordeum vulgare |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.2.3.4 |
additional information |
for wild type oxalate oxidase, glycolate does not serve as a substrate and does not significantly inhibit turnover when included in the assay at equimolar (20mM) concentrations of oxalate |
Hordeum vulgare |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.2.3.4 |
NaCl |
specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NH4Cl |
Hordeum vulgare |
|
1.2.3.4 |
NH4Cl |
specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NaCl |
Hordeum vulgare |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.2.3.4 |
Hordeum vulgare |
P45850 |
barley |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.2.3.4 |
mutant proteins |
Hordeum vulgare |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.2.3.4 |
0.055 |
- |
asparagine mutant (N85A), thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is higher in the presence of 1 M NH4Cl or 1 M NaCl and slightly lower when 1 M formamide is added |
Hordeum vulgare |
1.2.3.4 |
0.24 |
- |
asparagine mutant (N75A),thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is slightly higher in the presence of 1 M NH4Cl or 1 M NaCl and does not change when 1 M formamide is added |
Hordeum vulgare |
1.2.3.4 |
10.26 |
- |
wild type, thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4 |
Hordeum vulgare |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.2.3.4 |
additional information |
glycolate does not serve as a substrate |
Hordeum vulgare |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.2.3.4 |
OXO |
- |
Hordeum vulgare |