Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kupke, T.; Schwarz, W.
    4-Phosphopantetheine biosynthesis in archaea (2006), J. Biol. Chem., 281, 5435-5444.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.36 expressed in Escherichia coli strain M15 Methanocaldococcus jannaschii
6.3.2.5 expressed in Escherichia coli M15 (pREP4) cells Methanocaldococcus jannaschii
6.3.2.5 overexpression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.36 C54A comparable activity with that of the wild type enzyme Methanocaldococcus jannaschii
4.1.1.36 C73A comparable activity with that of the wild type enzyme Methanocaldococcus jannaschii
4.1.1.36 C94A comparable activity with that of the wild type enzyme Methanocaldococcus jannaschii
4.1.1.36 C96A comparable activity with that of the wild type enzyme Methanocaldococcus jannaschii
4.1.1.36 E168A drastically reduced activity Methanocaldococcus jannaschii
4.1.1.36 E168D significantly decreased activity Methanocaldococcus jannaschii
4.1.1.36 E168K residual activity Methanocaldococcus jannaschii
4.1.1.36 H139A/E168K drastically reduced PPC decarboxylase activity Methanocaldococcus jannaschii
4.1.1.36 H87A reduced activity Methanocaldococcus jannaschii
4.1.1.36 H87N reduced activity Methanocaldococcus jannaschii
6.3.2.5 K310Q very low activity Methanocaldococcus jannaschii
6.3.2.5 K310Q the mutant has very low activity Methanocaldococcus jannaschii
6.3.2.5 N217H mutant enzyme has residual activity Methanocaldococcus jannaschii
6.3.2.5 N217H the mutant has only residual enzyme activity Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.36 24060
-
 10 * 24060, gel filtration Methanocaldococcus jannaschii
4.1.1.36 242000
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine Methanocaldococcus jannaschii
-
 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine Methanocaldococcus jannaschii DSM 2661
-
 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.36 Methanocaldococcus jannaschii
-
-
-
4.1.1.36 Methanocaldococcus jannaschii DSM 2661
-
-
-
6.3.2.5 Methanocaldococcus jannaschii Q58323
-
-
6.3.2.5 Methanocaldococcus jannaschii DSM 2661 Q58323
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.36 Ni-NTA resin chromatography and Superdex 200 gel filtration Methanocaldococcus jannaschii
6.3.2.5 Ni-NTA resin column chromatography Methanocaldococcus jannaschii
6.3.2.5 purified as His tag protein Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.36 N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 Methanocaldococcus jannaschii pantotheine 4'-phosphate + CO2
-
 ?
4.1.1.36 N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 Methanocaldococcus jannaschii DSM 2661 pantotheine 4'-phosphate + CO2
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Methanocaldococcus jannaschii CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine detection of the 4'-phosphopantothenoyl-CMP intermediate Methanocaldococcus jannaschii CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Methanocaldococcus jannaschii DSM 2661 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine detection of the 4'-phosphopantothenoyl-CMP intermediate Methanocaldococcus jannaschii DSM 2661 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.36 homodecamer 10 * 24060, gel filtration Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.1.1.36 CoaC part of the bifunctional Dfp protein Methanocaldococcus jannaschii
4.1.1.36 PPC decarboxylase
-
 Methanocaldococcus jannaschii
6.3.2.5 CoaB
-
 Methanocaldococcus jannaschii
6.3.2.5 Dfp
-
 Methanocaldococcus jannaschii
6.3.2.5 phosphopantothenate-cysteine ligase
-
 Methanocaldococcus jannaschii
6.3.2.5 PPCS
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.36 flavin
-
 Methanocaldococcus jannaschii
6.3.2.5 CTP
-
 Methanocaldococcus jannaschii