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Literature summary extracted from

  • Smal, C.; Vertommen, D.; Bertrand, L.; Ntamashimikiro, S.; Rider, M.H.; Van Den Neste, E.; Bontemps, F.
    Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity (2006), J. Biol. Chem., 281, 4887-4893.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 expressed as His-tag fusion protein in HEK293T cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.74 S11A no significant effect on activity Homo sapiens
2.7.1.74 S11E no significant effect on activity Homo sapiens
2.7.1.74 S15A no significant effect on activity Homo sapiens
2.7.1.74 S15E no significant effect on activity Homo sapiens
2.7.1.74 S74A mutation markedly decreases activity Homo sapiens
2.7.1.74 S74E mutation does not significantly modify dCK activity Homo sapiens
2.7.1.74 T3A no significant effect on activity Homo sapiens
2.7.1.74 T3E no significant effect on activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 lambda protein phosphatase
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.00016
-
 2'-deoxycytidine with ATP as cosubstrate Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 34000
-
 2 * 34000, SDS-PAGE, native mass by gel filtration Homo sapiens
2.7.1.74 68000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 additional information Homo sapiens catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy ?
-
 ?
2.7.1.74 NTP + 1-beta-D-arabinosylcytosine Homo sapiens i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies NDP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2',2'-difluorodeoxycytidine Homo sapiens i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2',3'-dideoxycytidine Homo sapiens i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections NDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2'-deoxy-3'-thiacytidine Homo sapiens i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2-chloro-2'-deoxyadenosine Homo sapiens i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 NTP + 9-beta-D-arabinosyl 2-fluoroadenine Homo sapiens i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyadenosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyguanosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens P27707
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.74 phosphoprotein at least four residues phosphorylated, Thr-3, Ser-11, Ser-15, and Ser-74, Ser-74 phosphorylation level important for enzyme activity Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 recombinant protein using His-tag Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 CCRF-CEM cell
-
 Homo sapiens
-
2.7.1.74 leukemia cell
-
 Homo sapiens
-
2.7.1.74 lymphoid cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 additional information catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy Homo sapiens ?
-
 ?
2.7.1.74 NTP + 1-beta-D-arabinosylcytosine i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies Homo sapiens NDP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2',2'-difluorodeoxycytidine i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors Homo sapiens NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2',3'-dideoxycytidine i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections Homo sapiens NDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2'-deoxy-3'-thiacytidine i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections Homo sapiens NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
2.7.1.74 NTP + 2-chloro-2'-deoxyadenosine i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies Homo sapiens NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 NTP + 9-beta-D-arabinosyl 2-fluoroadenine i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies Homo sapiens NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyadenosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 UTP + 2'-deoxyguanosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 dimer 2 * 34000, SDS-PAGE, native mass by gel filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens