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Literature summary extracted from

  • Faehnle, C.R.; Le Coq, J.; Liu, X.; Viola, R.E.
    Examination of key intermediates in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase from a gram-positive infectious bacteria (2006), J. Biol. Chem., 281, 31031-31040.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.11 medicine target for antibiotic development Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expression as His-tag fusion protein in Escherichia coli BL21 (DE3) Streptococcus pneumoniae
1.2.1.11 into a pET28a vector for expression in Escherichia coli BL21DE3 Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.11
-
 Streptococcus pneumoniae
1.2.1.11 crystallization of the apo-enzyme, in complex with NADP+, in complex with L-aspartate-beta-semialdehyde, in complex with NADP+ and L-aspartate-beta-semialdehyde Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.11 3.3
-
 NADPH
-
 Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Streptococcus pneumoniae
-
 L-4-aspartyl phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11 Ni-affinity chromatography and anion exchange chromatography Streptococcus pneumoniae
1.2.1.11 using a Co2+ affinity matrix and anion exchange chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH
-
 Streptococcus pneumoniae L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Streptococcus pneumoniae L-4-aspartyl phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.11 homodimer crystallization Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Streptococcus pneumoniae
1.2.1.11 aspartate-beta-semialdehyde dehydrogenase
-
 Streptococcus pneumoniae
1.2.1.11 aspartate-semialdehyde dehydrogenase
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.11 22
-
 activity assay performed at room temperature Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.11 2
-
 L-aspartate-semialdehyde
-
 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.11 9
-
 activity assay Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NAD+ in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ Streptococcus pneumoniae
1.2.1.11 NADP+
-
 Streptococcus pneumoniae
1.2.1.11 NADP+ in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+ Streptococcus pneumoniae
1.2.1.11 NADPH
-
 Streptococcus pneumoniae