BRENDA - Enzyme Database show

Examination of key intermediates in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase from a gram-positive infectious bacteria

Faehnle, C.R.; Le Coq, J.; Liu, X.; Viola, R.E.; J. Biol. Chem. 281, 31031-31040 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.2.1.11
medicine
target for antibiotic development
Streptococcus pneumoniae
Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expression as His-tag fusion protein in Escherichia coli BL21 (DE3); into a pET28a vector for expression in Escherichia coli BL21DE3
Streptococcus pneumoniae
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.2.1.11
; crystallization of the apo-enzyme, in complex with NADP+, in complex with L-aspartate-beta-semialdehyde, in complex with NADP+ and L-aspartate-beta-semialdehyde
Streptococcus pneumoniae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
3.3
-
NADPH
-
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Streptococcus pneumoniae
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Streptococcus pneumoniae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
Ni-affinity chromatography and anion exchange chromatography; using a Co2+ affinity matrix and anion exchange chromatography
Streptococcus pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
674667
Streptococcus pneumoniae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
674667
Streptococcus pneumoniae
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
crystallization
Streptococcus pneumoniae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
22
-
activity assay performed at room temperature
Streptococcus pneumoniae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
2
-
L-aspartate-semialdehyde
-
Streptococcus pneumoniae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
activity assay
Streptococcus pneumoniae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NAD+
in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+
Streptococcus pneumoniae
1.2.1.11
NADP+
; in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+
Streptococcus pneumoniae
1.2.1.11
NADPH
-
Streptococcus pneumoniae
Application (protein specific)
EC Number
Application
Commentary
Organism
1.2.1.11
medicine
target for antibiotic development
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expression as His-tag fusion protein in Escherichia coli BL21 (DE3); into a pET28a vector for expression in Escherichia coli BL21DE3
Streptococcus pneumoniae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NAD+
in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+
Streptococcus pneumoniae
1.2.1.11
NADP+
; in contrast to Gram-negative bacterial aspartate-beta-semialdehyde dehydrogenases which are specific for NADP+ the enzyme of Gram-positive showed a dual specificity for NAD+ and NADP+
Streptococcus pneumoniae
1.2.1.11
NADPH
-
Streptococcus pneumoniae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.2.1.11
; crystallization of the apo-enzyme, in complex with NADP+, in complex with L-aspartate-beta-semialdehyde, in complex with NADP+ and L-aspartate-beta-semialdehyde
Streptococcus pneumoniae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
3.3
-
NADPH
-
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Streptococcus pneumoniae
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
Ni-affinity chromatography and anion exchange chromatography; using a Co2+ affinity matrix and anion exchange chromatography
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
674667
Streptococcus pneumoniae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
674667
Streptococcus pneumoniae
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
crystallization
Streptococcus pneumoniae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
22
-
activity assay performed at room temperature
Streptococcus pneumoniae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
2
-
L-aspartate-semialdehyde
-
Streptococcus pneumoniae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
activity assay
Streptococcus pneumoniae