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Literature summary extracted from

  • Mahmood, N.A.; Biemans-Oldehinkel, E.; Patzlaff, J.S.; Schuurman-Wolters, G.K.; Poolman, B.
    Ion specificity and ionic strength dependence of the osmoregulatory ABC transporter OpuA (2006), J. Biol. Chem., 281, 29830-29839.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.9 dioleoyl-phosphatidylglycerol needed for full osmoregulatory activity Lactococcus lactis
7.6.2.9 Tetracaine instantaneously activates inside-out oriented OpuA, reconstitued in membranes with 40% of anionic lipid, 1mM equally effective than 0.2 mM KCl Lactococcus lactis

Application

EC Number Application Comment Organism
7.6.2.9 additional information the cystathionine beta-synthase module in OpuA constitutes the ionic strength sensor whose activity is modulated by the C-terminal anionic tail Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.9 additional information OpuAdelta12, anionic tail deletion mutant, Rb+ and Cs+ no longer inhibitory, more K+ needed for activation than for the wild-type Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.9 Cs+ potent inhibitor Lactococcus lactis
7.6.2.9 Rb+ potent inhibitor Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.9 membrane
-
 Lactococcus lactis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.9 Ba2+ bivalent cations more effective activators than monovalent cations Lactococcus lactis
7.6.2.9 K+ maximal enzyme activity reached with 250 mM KCl, no further effect in the presence of the protonophore SF6847 Lactococcus lactis
7.6.2.9 Li+
-
 Lactococcus lactis
7.6.2.9 Mg2+ bivalent cations more effective activators than monovalent cations Lactococcus lactis
7.6.2.9 Na+
-
 Lactococcus lactis
7.6.2.9 NH4+ less effective than KCl, in the presence of the protonophore SF6847 much more effective Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.9 Lactococcus lactis Q9KIF7 strain Opu401
-
7.6.2.9 Lactococcus lactis IL1403 Q9KIF7 strain Opu401
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.9 by Ni2+-nitrilotriacetic acid resin Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Lactococcus lactis IL1403 ADP + phosphate + glycine betaine/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.9 OpuA
-
 Lactococcus lactis