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Literature summary extracted from

  • Watanabe, S.; Kodaki, T.; Makino, K.
    A novel alpha-ketoglutaric semialdehyde dehydrogenase: evolutionary insight into an alternative pathway of bacterial L-arabinose metabolism (2006), J. Biol. Chem., 281, 28876-28888.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.24 expression in Escherichia coli as His-tag fusion protein Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.24 0.011
-
 alpha-Ketoglutaric semialdehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C Azospirillum brasilense
1.2.1.24 0.0159
-
 alpha-Ketoglutaric semialdehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NADP+. 25°C Azospirillum brasilense
1.2.1.24 0.0163
-
 Glutaraldehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C Azospirillum brasilense
1.2.1.24 0.0385
-
 succinate semialdehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.24 55000
-
 SDS-PAGE Azospirillum brasilense
1.2.1.24 220000
-
 gel-filtration Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.24 Azospirillum brasilense Q1JUP4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.24 Ni-affinity chromatography Azospirillum brasilense

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.24 cell culture
-
 Azospirillum brasilense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.24 acetaldehyde + NAD+ + H2O
-
 Azospirillum brasilense acetate + NADH + H+
-
 ?
1.2.1.24 alpha-ketoglutaric semialdehyde + NAD+ + H2O
-
 Azospirillum brasilense alpha-ketoglutarate + NADH
-
 ?
1.2.1.24 alpha-ketoglutaric semialdehyde + NADP+ + H2O
-
 Azospirillum brasilense alpha-ketoglutarate + NADPH
-
 ?
1.2.1.24 betaine aldehyde + NAD+ + H2O
-
 Azospirillum brasilense 2-trimethylammonioacetate + NADH
-
 ?
1.2.1.24 butanaldehyde + NAD+ + H2O
-
 Azospirillum brasilense butanoate + NADH
-
 ?
1.2.1.24 formaldehyde + NAD+ + H2O
-
 Azospirillum brasilense formate + NADH
-
 ?
1.2.1.24 glutaraldehyde + NAD+ + H2O
-
 Azospirillum brasilense glutarate + NADH
-
 ?
1.2.1.24 glyceraldehyde + NAD+ + H2O
-
 Azospirillum brasilense glycerate + NADH
-
 ?
1.2.1.24 glycoaldehyde + NAD+ + H2O
-
 Azospirillum brasilense glycolate + NADH
-
 ?
1.2.1.24 heptanaldehyde + NAD+ + H2O
-
 Azospirillum brasilense heptanoate + NADH
-
 ?
1.2.1.24 hexanaldehyde + NAD+ + H2O
-
 Azospirillum brasilense hexanoate + NADH
-
 ?
1.2.1.24 isobutyraldehyde + NAD+ + H2O
-
 Azospirillum brasilense isobutyrate + NADH + H+
-
 ?
1.2.1.24 octanaldehyde + NAD+ + H2O
-
 Azospirillum brasilense octanoate + NADH
-
 ?
1.2.1.24 pentanaldehyde + NAD+ + H2O
-
 Azospirillum brasilense pentanoate + NADH
-
 ?
1.2.1.24 propanaldehyde + NAD+ + H2O
-
 Azospirillum brasilense propanoate + NADH
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Azospirillum brasilense succinate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.24 tetramer 4 * 55000, gel-filtration, SDS-PAGE Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
1.2.1.24 alphaKGSA dehydrogenase
-
 Azospirillum brasilense
1.2.1.24 SSALDH
-
 Azospirillum brasilense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.24 12.3
-
 Glutaraldehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C Azospirillum brasilense
1.2.1.24 12.3
-
 alpha-Ketoglutaric semialdehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NADP+. 25°C Azospirillum brasilense
1.2.1.24 31.7
-
 succinate semialdehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C Azospirillum brasilense
1.2.1.24 51.2
-
 alpha-Ketoglutaric semialdehyde 1 mM EDTA, 1 mM 2-mercaptoethanol, 66.7 mM potassium phosphate pH 7.2, 1.5 mM NAD+. 25°C Azospirillum brasilense