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Literature summary extracted from

  • Flemming, D.; Hellwig, P.; Lepper, S.; Kloer, D.P.; Friedrich, T.
    Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I) (2006), J. Biol. Chem., 281, 24781-24789.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.2 D115N 45% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 D146N 59% of deamino-NADH oxidase activity of the wild type enzyme, one fourth reduced complex 1 content Escherichia coli
7.1.1.2 D152N 59% of deamino-NADH oxidase activity of the wild type enzyme, one third reduced complex 1 content Escherichia coli
7.1.1.2 D77E 54% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 D77N completely abolished electron transfer activity, 12% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 D94E 83% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 D94N completely abolished electron transfer activity, 12% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 E119Q 88% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 E163Q 76% of deamino-NADH oxidase activity of the wild type enzyme, one third reduced complex 1 content Escherichia coli
7.1.1.2 E67D 78% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
7.1.1.2 E67Q completely abolished electron transfer activity, one fourth reduced complex 1 content, 10% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 Piericidin A
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 Fe-S contains 9 iron-sulfur clusters Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Escherichia coli
-
-
-
7.1.1.2 Escherichia coli ANN023
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 Source 15Q column chromatography and Sephacryl S-300 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 2 ferricyanide + deamino-NADH
-
 Escherichia coli 2 ferrocyanide + deamino-NAD+ + H+
-
 ?
7.1.1.2 2 ferricyanide + deamino-NADH
-
 Escherichia coli ANN023 2 ferrocyanide + deamino-NAD+ + H+
-
 ?
7.1.1.2 2 ferricyanide + NADH
-
 Escherichia coli 2 ferrocyanide + NAD+ + H+
-
 ?
7.1.1.2 2 ferricyanide + NADH
-
 Escherichia coli ANN023 2 ferrocyanide + NAD+ + H+
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Escherichia coli NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Escherichia coli ANN023 NAD+ + ubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 tridecamer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Escherichia coli
7.1.1.2 NADH:ubiquinone oxidoreductase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FMN
-
 Escherichia coli
7.1.1.2 NADH
-
 Escherichia coli