Literature summary extracted from

Turanov, A.A.; Su, D.; Gladyshev, V.N.
Characterization of alternative cytosolic forms and cellular targets of mouse mitochondrial thioredoxin reductase (2006), J. Biol. Chem., 281, 22953-22963.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.8.1.9	EDTA	if the concentration of EDTA in the reaction mixture is higher than 0.1 mM the enzyme shows full activity	Mus musculus
1.8.1.9	EDTA	if the concentration of EDTA in the reaction mixture is higher than 0.1 mM the enzyme shows full activity	Homo sapiens

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.8.1.9	expressed in Escherichia coli BL21(DE3) cells	Mus musculus
1.8.1.9	expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.8.1.9	additional information	no inhibition by Ca2+ or Co2+	Homo sapiens
1.8.1.9	additional information	no inhibition by Ca2+ or Co2+	Mus musculus
1.8.1.9	Zn2+	0.1-0.2 mM and above	Homo sapiens
1.8.1.9	Zn2+	0.1-0.2 mM and above	Mus musculus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8.1.9	0.00141	-	thioredoxin	rat thioredoxin as substrate	Homo sapiens
1.8.1.9	0.00157	-	thioredoxin	Escherichia coli thioredoxin as substrate	Homo sapiens
1.8.1.9	0.00183	-	thioredoxin	human thioredoxin as substrate	Homo sapiens
1.8.1.9	0.00236	-	thioredoxin	human thioredoxin as substrate	Mus musculus
1.8.1.9	0.00256	-	thioredoxin	Escherichia coli thioredoxin as substrate	Mus musculus
1.8.1.9	0.0051	-	thioredoxin	rat thioredoxin as substrate	Mus musculus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.8.1.9	cytosol	-	Homo sapiens	5829	-
1.8.1.9	cytosol	TR1	Mus musculus	5829	-
1.8.1.9	mitochondrion	TR3	Mus musculus	5739	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.1.9	Homo sapiens	-	-	-
1.8.1.9	Mus musculus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.8.1.9	Sepharose affinity column chromatography	Homo sapiens
1.8.1.9	TALONTM column chromatography and Sepharose affinity column chromatography	Mus musculus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.8.1.9	liver	-	Mus musculus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.1.9	additional information	TR3 does not have catalytic preferences for mitochondrial thioredoxin versus cytosolic thioredoxin	Mus musculus	?	-	?
1.8.1.9	thioredoxin + NADP+	-	Mus musculus	thioredoxin disulfide + NADPH	-	?
1.8.1.9	thioredoxin + NADP+	-	Homo sapiens	thioredoxin disulfide + NADPH	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.8.1.9	thioredoxin reductase	-	Mus musculus
1.8.1.9	thioredoxin reductase	-	Homo sapiens
1.8.1.9	TR1	-	Homo sapiens
1.8.1.9	TR3	-	Mus musculus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.8.1.9	0.016	-	thioredoxin	Escherichia coli thioredoxin as substrate	Homo sapiens
1.8.1.9	3.5	-	thioredoxin	human thioredoxin as substrate	Mus musculus
1.8.1.9	8.1	-	thioredoxin	human thioredoxin as substrate	Homo sapiens
1.8.1.9	19.97	-	thioredoxin	human thioredoxin as substrate	Mus musculus
1.8.1.9	25.78	-	thioredoxin	human thioredoxin as substrate	Homo sapiens
1.8.1.9	27.4	-	thioredoxin	rat thioredoxin as substrate	Homo sapiens
1.8.1.9	29.5	-	thioredoxin	Escherichia coli thioredoxin as substrate	Mus musculus
1.8.1.9	37.88	-	thioredoxin	rat thioredoxin as substrate	Mus musculus
1.8.1.9	46.57	-	thioredoxin	Escherichia coli thioredoxin as substrate	Homo sapiens

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Release 2023.1 (January 2023)