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Literature summary extracted from

  • Bieganowski, P.; Seidle, H.F.; Wojcik, M.; Brenner, C.
    Synthetic lethal and biochemical analyses of NAD and NADH kinases in Saccharomyces cerevisiae establish separation of cellular functions (2006), J. Biol. Chem., 281, 22439-22445.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23 expression in Escherichia coli as N-terminally His-tagged fusion Saccharomyces cerevisiae
2.7.1.86 expressed in Escherichia coli Saccharomyces cerevisiae
2.7.1.86 expression in Escherichia coli as N-terminally His-tagged fusion Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.23 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
2.7.1.23 cytosol
-
 Saccharomyces cerevisiae 5829
-
2.7.1.86 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
2.7.1.86 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.23 ATP + NAD+ Saccharomyces cerevisiae Utr1 is responsible for essentially all of the NAD/NADH kinase activity resident in the cytoplasm ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ Saccharomyces cerevisiae Yef1 contributes very slightly to total NAD and NADH kinase activities in vivo ADP + NADP+
-
 ?
2.7.1.86 ATP + NADH Saccharomyces cerevisiae Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants ADP + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Saccharomyces cerevisiae P21373
-
-
2.7.1.23 Saccharomyces cerevisiae P32622
-
-
2.7.1.86 Saccharomyces cerevisiae
-
-
-
2.7.1.86 Saccharomyces cerevisiae Q06892
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23
-
 Saccharomyces cerevisiae
2.7.1.86
-
 Saccharomyces cerevisiae
2.7.1.86 cobalt chelate affinity column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.86 0.1
-
 with GTP as phosphate donor Saccharomyces cerevisiae
2.7.1.86 0.5
-
 with CTP as phosphate donor Saccharomyces cerevisiae
2.7.1.86 1.2
-
 with ATP as phosphate donor Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+ Utr1 is responsible for essentially all of the NAD/NADH kinase activity resident in the cytoplasm Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ Yef1 contributes very slightly to total NAD and NADH kinase activities in vivo Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ specific activity is 43fold higher than with NADH and ATP Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ specific activity is about 3fold higher than with NADH Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.23 ATP + NADH specific activity is 43fold lower than with NAD+ and ATP Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH specific activity is about 3fold lower than with NAD+ and ATP Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.23 CTP + NAD+ specific activity is 16.5fold lower than with NAD+ and ATP Saccharomyces cerevisiae CDP + NADP+
-
 ?
2.7.1.23 CTP + NAD+ specific activity is 7fold lower than with NAD+ and ATP Saccharomyces cerevisiae CDP + NADP+
-
 ?
2.7.1.23 CTP + NADH specific activity is 13.2fold lower than with NADH and ATP Saccharomyces cerevisiae CDP + NADPH
-
 ?
2.7.1.23 CTP + NADH specific activity is 2.4fold lower than with NADH and ATP Saccharomyces cerevisiae CDP + NADPH
-
 ?
2.7.1.23 dATP + NAD+ specific activity is 2.6fold lower than with ATP and NAD+ Saccharomyces cerevisiae dADP + NADP+
-
 ?
2.7.1.23 dATP + NAD+ specific activity is similar to reaction with ATP and NAD+ Saccharomyces cerevisiae dADP + NADP+
-
 ?
2.7.1.23 dCTP + NAD+ specific activity is 11fold lower than with ATP and NAD+ Saccharomyces cerevisiae dCDP + NADP+
-
 ?
2.7.1.23 dCTP + NAD+ specific activity is 3.3fold lower than with ATP and NAD+ Saccharomyces cerevisiae dCDP + NADP+
-
 ?
2.7.1.23 dGTP + NAD+ specific activity is 11fold lower than with ATP and NAD+ Saccharomyces cerevisiae dGDP + NADP+
-
 ?
2.7.1.23 dGTP + NAD+ specific activity is 13.2fold lower than with ATP and NAD+ Saccharomyces cerevisiae dGDP + NADP+
-
 ?
2.7.1.86 ATP + NAD+
-
 Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.86 ATP + NADH
-
 Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH phosphorylates NADH with 2% of the specific activity of its NAD kinase activity Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH possesses 33% activity with NADH and ATP compared to the activity obtained with NAD and ATP Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants Saccharomyces cerevisiae ADP + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 NAD kinase
-
 Saccharomyces cerevisiae
2.7.1.23 Utr
-
 Saccharomyces cerevisiae
2.7.1.23 YEF1 low specific activity cytosolic NAD kinase Saccharomyces cerevisiae
2.7.1.86 NADH kinase POS5, mitochondrial
-
 Saccharomyces cerevisiae
2.7.1.86 Pos5
-
 Saccharomyces cerevisiae
2.7.1.86 Utr1
-
 Saccharomyces cerevisiae
2.7.1.86 YEF1
-
 Saccharomyces cerevisiae
2.7.1.86 YEL041W
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.86 ATP
-
 Saccharomyces cerevisiae