EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.29 | Tween 20 | at 0.2% | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.29 | expression of N-terminally His-tagged enzyme in Escherichia coli | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.29 | native protein, 20°C, sitting drop vapour diffusion mthod, mixing of 200 nl of 90 mg/ml protein in 10 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol with 100 nl of precipitant solution consisting of 25% PEG 3350, 200 mM magnesium formate, pH 5.5, and equilibration against 0.1 ml of the precipitant solution, selenomethionine-labeled protein by suspending a 0.003 ml drop containing 26 mg/ml protein, 333 mM NaCl, 0.67 mM MgCl2, 0.67 mM GGPP, 3% glycerol, 15% 2-methyl-2,4-pentanediol, 1.7% PEG 10,000, 6.7 mM HEPES, pH 7.5, over a 1 ml reservoir containing 45% 2-methyl-2,4-pentanediol, and 5% PEG 10000, X-ray diffraction structure determination and analysis at 2.7-2.8 A resolution | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.29 | EDTA | - |
Homo sapiens | |
2.5.1.29 | geranylgeranyl diphosphate | competitive product inhibition | Homo sapiens | |
2.5.1.29 | nitrogen-containing bisphosphonates | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | additional information | - |
additional information | steady-state kinetics, recombinant enzyme | Homo sapiens | |
2.5.1.29 | 0.003 | - |
isopentenyl diphosphate | pH 7.7, 37°C, recombinant enzyme | Homo sapiens | |
2.5.1.29 | 0.0042 | - |
trans,trans-farnesyl diphosphate | pH 7.7, 37°C, recombinant enzyme | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.29 | Mg2+ | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 200000 | - |
about, gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | trans,trans-farnesyl diphosphate + isopentenyl diphosphate | Homo sapiens | the enzyme takes part in the mevalonate pathway of isoprenoid synthesis, and plays a crucial role in cell survival, overview | diphosphate + geranylgeranyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.29 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.29 | recombinant N-terminally His-tagged enzyme from Escherichia coli, the His-tag is cleaved of by TEV | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | trans,trans-farnesyl diphosphate + isopentenyl diphosphate | the enzyme takes part in the mevalonate pathway of isoprenoid synthesis, and plays a crucial role in cell survival, overview | Homo sapiens | diphosphate + geranylgeranyl diphosphate | - |
? | |
2.5.1.29 | trans,trans-farnesyl diphosphate + isopentenyl diphosphate | tight binding substrate and product structures, overview | Homo sapiens | diphosphate + geranylgeranyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.29 | hexamer | three dimers join together to form a propeller-bladed hexameric molecule, quaternary structure, overview | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.29 | geranylgeranyl pyrophosphate synthase | - |
Homo sapiens |
2.5.1.29 | GGPS | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | 0.204 | - |
trans,trans-farnesyl diphosphate | pH 7.7, 37°C, recombinant enzyme | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 7.7 | - |
assay at | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | 0.025 | - |
geranylgeranyl diphosphate | pH 7.7, 37°C, recombinant enzyme, versus trans,trans-farnesyl diphosphate | Homo sapiens |