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Literature summary extracted from

  • Nakanishi, H.; Shindou, H.; Hishikawa, D.; Harayama, T.; Ogasawara, R.; Suwabe, A.; Taguchi, R.; Shimizu, T.
    Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production (2006), J. Biol. Chem., 281, 20140-20147.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.23 LPCAT1, DNA and amino acid sequence determination and analysis Rattus norvegicus
2.3.1.23 LPCAT1, DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of FLAG-tagged enzyme in CHO-K1 cells, subcellular distribution pattern analysis shows enzyme expression mainly in the endoplasmic reticulum-Golgi, but also in mitochondria, and lysosome of the transfected cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.23 palmitoyl-CoA inhibition at higher concentrations of palmitoyl-CoA, possibly due to a detergent effect Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.23 additional information
-
 additional information kinetics Mus musculus
2.3.1.23 0.0023
-
 1-palmitoyl-lysophosphatidylcholine pH 7.4, 30°C, recombinant enzyme Mus musculus
2.3.1.23 0.003
-
 palmitoyl-CoA pH 7.4, 30°C, recombinant enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.23 membrane the enzyme contains 3 transmembrane domains Mus musculus 16020
-
2.3.1.23 membrane the enzyme contains 3 transmembrane domains Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.23 additional information Ca2+ is not required Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.23 60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 additional information Mus musculus the enzyme synthesizes phosphatidylcholine in pulmonary surfactant and plays a pivotal role in respiratory physiology and in membrane biogenesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Mus musculus Q3TFD2
-
-
2.3.1.23 Rattus norvegicus Q1HAQ0 Sprague-Dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 alveolar type II cell
-
 Mus musculus
-
2.3.1.23 alveolar type II cell
-
 Rattus norvegicus
-
2.3.1.23 brain
-
 Mus musculus
-
2.3.1.23 epididymis
-
 Mus musculus
-
2.3.1.23 lung
-
 Rattus norvegicus
-
2.3.1.23 lung LPCAT1 is predominantly expressed in the lung, in particular in alveolar type II cells, but occurs also in other tissues Mus musculus
-
2.3.1.23 additional information LPCAT1 shows a wide tissue distribution, overview Mus musculus
-
2.3.1.23 ovary
-
 Mus musculus
-
2.3.1.23 placenta
-
 Mus musculus
-
2.3.1.23 spleen
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 additional information the enzyme synthesizes phosphatidylcholine in pulmonary surfactant and plays a pivotal role in respiratory physiology and in membrane biogenesis Mus musculus ?
-
 ?
2.3.1.23 additional information LPCAT1 shows a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl- or 1-palmitoyl-LPC as acyl donors and acceptors, respectively Mus musculus ?
-
 ?
2.3.1.23 palmitoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine i.e. 1-palmitoyl-lysophosphatidylcholine Mus musculus CoA + dipalmitoylphosphatidylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.23 ? x * 60000, recombinant enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 LPCAT1
-
 Mus musculus
2.3.1.23 LPCAT1
-
 Rattus norvegicus
2.3.1.23 lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1
-
 Mus musculus
2.3.1.23 lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 39
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.23 7.4 10 recombinant enzyme Mus musculus