EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.14 | expression of GST-tagged enzyme peptide fragments, expression of residues 1562 of rhodopsin kinase in Spodoptera frugiperda Sf9 cells, expression of the truncation mutant comprising residues 32562 in Escherichia coli as C-terminally His6-tagged protein | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.11.14 | A11R | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | D2A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | E7A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | F15A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | F3A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | G4A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | L6A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | additional information | a kinase mutant lacking the N-terminal recoverin binding site is unable to phosphorylate light-activated rhodopsin | Bos taurus |
2.7.11.14 | N12A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | S13A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | S5A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | T8A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | V10A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
2.7.11.14 | V9A | site-directed mutagenesis, the mutation of the residues leads to alterd binding of recoverin to the N-terminal fragment compared to the wild-type enzyme, kinetics constant, overview | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | recoverin | binds exclusively to an amphipathic peptide at the N-terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase, calcium depletion causes release of recoverin from rhodopsin kinase, freeing the kinase to phosphorylate rhodopsin and to terminate the light response | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.14 | membrane | Ca2+-bound enzyme | Bos taurus | 16020 | - |
2.7.11.14 | soluble | Ca2+-free enzyme | Bos taurus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | Ca2+ | induces a conformational change, while in the calciumfree form, the myristoyl group is held within a hydrophobic cleft, addition of calcium leads to its release and to membrane binding, inhibits rhodopsin binding to the enzyme via recoverin, which binds to the amphipathic peptide at the N-terminus of the enzyme and blocks rhodopsin binding | Bos taurus | |
2.7.11.14 | Mg2+ | - |
Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.14 | Bos taurus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.14 | lipoprotein | the enzyme is myristoylated in a Ca2+-dependent manner | Bos taurus |
2.7.11.14 | phosphoprotein | the enzyme performs autophosphorylation in a light-dependent manner | Bos taurus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.14 | recombinant GST-tagged enzyme peptide fragments by glutathione affinity chromatography and gel filtration, recombinant His6-tagged truncation mutant 32562 from Escherichia coli by nickel affinity chromatography and gel filtration, recombinant enzyme N-terminal fragment from Spodoptera frugiperda Sf9 cells by recoverin affinity chromatography and gel filtration to homogeneity | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.14 | eye | - |
Bos taurus | - |
2.7.11.14 | retina | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | rhodopsin in rod outer segment membranes | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | additional information | the enzyme performs autophosphorylation in a light-dependent manner | Bos taurus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.14 | monomer | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 25 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 7.4 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | ATP | - |
Bos taurus |