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Literature summary extracted from

  • Combe, J.P.; Basran, J.; Hothi, P.; Leys, D.; Rigby, S.E.; Munro, A.W.; Scrutton, N.S.
    Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis (2006), J. Biol. Chem., 281, 17977-17988.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.14 K48A KM-value for dihydroorotate is 2.2fold higher than wild-type value, kcat is 411fold lower than wild-type value Lactococcus lactis
1.3.1.14 K48E KM-value for dihydroorotate is 4.1fold higher than wild-type value, kcat is 67.5fold lower than wild-type value Lactococcus lactis
1.3.1.14 K48Q KM-value for dihydroorotate is 1.5fold higher than wild-type value, kcat is 448fold lower than wild-type value Lactococcus lactis
1.3.1.14 K48R KM-value for dihydroorotate is 2fold lower than wild-type value, kcat is 117fold lower than wild-type value Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.14 0.0037
-
 NADH wild-type enzyme Lactococcus lactis
1.3.1.14 0.0144
-
 Orotate mutant enzyme K48Q Lactococcus lactis
1.3.1.14 0.0213
-
 Orotate wild-type enzyme Lactococcus lactis
1.3.1.14 0.044
-
 (S)-dihydroorotate mutant enzyme K48R Lactococcus lactis
1.3.1.14 0.062
-
 NAD+ wild-type enzyme Lactococcus lactis
1.3.1.14 0.09
-
 (S)-dihydroorotate wild-type enzyme Lactococcus lactis
1.3.1.14 0.131
-
 (S)-dihydroorotate mutant enzyme K48Q Lactococcus lactis
1.3.1.14 0.195
-
 (S)-dihydroorotate mutant enzyme K48A Lactococcus lactis
1.3.1.14 0.367
-
 (S)-dihydroorotate mutant enzyme K48E Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.14 Lactococcus lactis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.14 (S)-dihydroorotate + NAD+
-
 Lactococcus lactis orotate + NADH + H+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.14 0.021
-
 NADH mutant enzyme K48Q Lactococcus lactis
1.3.1.14 0.021
-
 Orotate mutant enzyme K48Q Lactococcus lactis
1.3.1.14 0.11
-
 (S)-dihydroorotate mutant enzyme K48Q Lactococcus lactis
1.3.1.14 0.12
-
 (S)-dihydroorotate mutant enzyme K48A Lactococcus lactis
1.3.1.14 0.42
-
 (S)-dihydroorotate mutant enzyme K48R Lactococcus lactis
1.3.1.14 0.73
-
 (S)-dihydroorotate mutant enzyme K48E Lactococcus lactis
1.3.1.14 25.7
-
 NADH wild-type enzyme Lactococcus lactis
1.3.1.14 25.7
-
 Orotate wild-type enzyme Lactococcus lactis
1.3.1.14 49.3
-
 (S)-dihydroorotate wild-type enzyme Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.14 4Fe-4S-center
-
 Lactococcus lactis
1.3.1.14 FAD
-
 Lactococcus lactis
1.3.1.14 FMN key charge-stabilizing role for Lys-48 of subunit D during reduction of FMN by dihydroorotate or by electron transfer from the 2Fe-2S center Lactococcus lactis