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Literature summary extracted from

  • Yano, T.; Li, L.S.; Weinstein, E.; Teh, J.S.; Rubin, H.
    Steady-state kinetics and inhibitory action of antitubercular phenothiazines on Mycobacterium tuberculosis type-II NADH-menaquinone oxidoreductase (NDH-2) (2006), J. Biol. Chem., 281, 11456-11463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.2 expressed in Mycobacterium smegmatis Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 dodecyl-beta-D-maltoside leads to immediate loss of activity Mycobacterium tuberculosis
7.1.1.2 Trifluoperazine 0.05 mM Mycobacterium tuberculosis
7.1.1.2 Triton X-100 leads to immediate loss of activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.2 50000
-
 calculated from sequence of cDNA Mycobacterium tuberculosis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
7.1.1.2 Triton X-100 leads to immediate loss of activity Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 ultracentrifugation Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
7.1.1.2 -80°C, alkaline buffer with 40% (w/v) glycerol, one month, without loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 additional information does not react with NADPH or oxygen Mycobacterium tuberculosis ?
-
 ?
7.1.1.2 NADH + 2-methyl-1,4-naphthoquinone
-
 Mycobacterium tuberculosis NAD+ + 2-methyl-1,4-naphthoquinol
-
 ?
7.1.1.2 NADH + decylubiquinone
-
 Mycobacterium tuberculosis NAD+ + decylubiquinol
-
 ?
7.1.1.2 NADH + H+ + 1,4-naphthoquinone
-
 Mycobacterium tuberculosis NAD+ + 1,4-naphthoquinol
-
 ?
7.1.1.2 NADH + menaquinone
-
 Mycobacterium tuberculosis NAD+ + menaquinol
-
 ?
7.1.1.2 NADH + phylloquinone
-
 Mycobacterium tuberculosis NAD+ + phylloquinol
-
 ?
7.1.1.2 NADH + ubiquinone-1
-
 Mycobacterium tuberculosis NAD+ + ubiquinol-1
-
 ?
7.1.1.2 NADH + ubiquinone-2
-
 Mycobacterium tuberculosis NAD+ + ubiquinol-2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 NDH-2
-
 Mycobacterium tuberculosis
7.1.1.2 type-II NADH-menaquinone oxidoreductase
-
 Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
7.1.1.2 8
-
 NDH-2 activity increases rapidly at pH 8.0 or above Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FAD
-
 Mycobacterium tuberculosis
7.1.1.2 NADH
-
 Mycobacterium tuberculosis