Literature summary extracted from
Oldham, M.L.; Brash, A.R.; Newcomer, M.E.
Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality (2005), J. Biol. Chem., 280, 39545-39552.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.40 |
expressed in Escherichia coli BL21(DE3) cells |
Plexaura homomalla |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.13.11.40 |
hanging drop vapour diffusion in 6.25% polyethylene glycol 8000, 100 mM imidazole acetate (pH 8.0), 100 mM CaCl2, and 5% sucrose at 22°C |
Plexaura homomalla |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.11.40 |
D39A |
118% activity compared to the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding |
Plexaura homomalla |
1.13.11.40 |
D39A/E47A |
106% activity compared to the wild type enzyme, a double mutant with calcium-binding residues from two of the three sites mutated exhibits no fluorescence resonance energy transfer signal |
Plexaura homomalla |
1.13.11.40 |
E47A |
65% of the activity of the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding |
Plexaura homomalla |
1.13.11.40 |
W41A |
140% activity compared to the wild type enzyme, exhibits only less than 2% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal |
Plexaura homomalla |
1.13.11.40 |
W77A |
44% of the activity of the wild type enzyme, exhibits only 4% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal |
Plexaura homomalla |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.40 |
Ca2+ |
Ca2+-regulated enzyme activity |
Plexaura homomalla |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.40 |
Plexaura homomalla |
- |
Caribbean sea whip coral |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.13.11.40 |
nickel-nitrilotriacetic acid-agarose chromatography, DE52 column chromatography, and Mono Q column chromatography |
Plexaura homomalla |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.40 |
arachidonic acid + O2 |
- |
Plexaura homomalla |
(8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.40 |
8R-lipoxygenase |
- |
Plexaura homomalla |
1.13.11.40 |
8R-LOX |
- |
Plexaura homomalla |