Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rajashankar, K.R.; Bryk, R.; Kniewel, R.; Buglino, J.A.; Nathan, C.F.; Lima, C.D.
    Crystal structure and functional analysis of lipoamide dehydrogenase from Mycobacterium tuberculosis (2005), J. Biol. Chem., 280, 33977-33983.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.4 hanging drop vapour diffusion method Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.4 D5A 4.3% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 E91A 49.6% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 E91K 62.5% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 F464A 5% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 H386A 20% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 H386K 9% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 H460E 69.4% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 H98A 3.3% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K103E 17.7% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K105A 44.7% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K216A 58.3% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K220A 81.2% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K223A 67.1% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K223E 70.5% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K224A 55.2% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K376A 52.2% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K67A 67.3% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K67E 64.3% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 K88E 74.8% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 N43A 11% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 R93A 6.5% activity of the wild type enzyme Mycobacterium tuberculosis
1.8.1.4 R93E 3.6% activity of the wild type enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Mycobacterium tuberculosis P9WHH9
-
-
1.8.1.4 Mycobacterium tuberculosis H37Rv P9WHH9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 metal-affinity chromatography and Superdex 200 gel filtration chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
Mycobacterium tuberculosis lipoamide + NADH + H+
-
?
1.8.1.4 dihydrolipoamide + NAD+
-
Mycobacterium tuberculosis H37Rv lipoamide + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 LDH
-
Mycobacterium tuberculosis
1.8.1.4 lipoamide dehydrogenase
-
Mycobacterium tuberculosis
1.8.1.4 LPD
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NAD+
-
Mycobacterium tuberculosis