EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.4 | expressed in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.1.4 | hanging drop vapour diffusion method | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.4 | D5A | 4.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | E91A | 49.6% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | E91K | 62.5% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | F464A | 5% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | H386A | 20% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | H386K | 9% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | H460E | 69.4% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | H98A | 3.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K103E | 17.7% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K105A | 44.7% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K216A | 58.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K220A | 81.2% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K223A | 67.1% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K223E | 70.5% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K224A | 55.2% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K376A | 52.2% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K67A | 67.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K67E | 64.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | K88E | 74.8% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | N43A | 11% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | R93A | 6.5% activity of the wild type enzyme | Mycobacterium tuberculosis |
1.8.1.4 | R93E | 3.6% activity of the wild type enzyme | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.4 | Mycobacterium tuberculosis | P9WHH9 | - |
- |
1.8.1.4 | Mycobacterium tuberculosis H37Rv | P9WHH9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.4 | metal-affinity chromatography and Superdex 200 gel filtration chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | dihydrolipoamide + NAD+ | - |
Mycobacterium tuberculosis | lipoamide + NADH + H+ | - |
? | |
1.8.1.4 | dihydrolipoamide + NAD+ | - |
Mycobacterium tuberculosis H37Rv | lipoamide + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.4 | LDH | - |
Mycobacterium tuberculosis |
1.8.1.4 | lipoamide dehydrogenase | - |
Mycobacterium tuberculosis |
1.8.1.4 | LPD | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | NAD+ | - |
Mycobacterium tuberculosis |