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Literature summary extracted from

  • Galkin, A.; Brandt, U.
    Superoxide radical formation by pure complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica (2005), J. Biol. Chem., 280, 30129-30135.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.2 R141M reduced activity compared to wild type enzyme Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 2-decyl-4-quinazolinyl amine 0.00022 mM Yarrowia lipolytica
7.1.1.2 antimycin A 0.0009 mM Yarrowia lipolytica
7.1.1.2 diphenyleineiodonium does not inhibit superoxide generation Yarrowia lipolytica
7.1.1.2 rotenone does not inhibit superoxide generation Yarrowia lipolytica
7.1.1.2 Sodium azide 10 mM Yarrowia lipolytica
7.1.1.2 Stigmatellin 0.001 mM Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion
-
 Yarrowia lipolytica 5739
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Yarrowia lipolytica
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 2.3
-
 mutant enzyme R141M, NADH-dependent activity of complex 1 containing proteoliposomes using n-decylubiquinone as substrate Yarrowia lipolytica
7.1.1.2 5.5
-
 wild type enzyme, NADH-dependent activity of complex 1 containing proteoliposomes using n-decylubiquinone as substrate Yarrowia lipolytica
7.1.1.2 16.1
-
 mutant enzyme R141M, NADH-dependent activity of complex 1 containing proteoliposomes using hexaammineruthenium-(III)-chloride as substrate Yarrowia lipolytica
7.1.1.2 23
-
 wild type enzyme, NADH-dependent activity of complex 1 containing proteoliposomes using hexaammineruthenium-(III)-chloride as substrate Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + hexaammineruthenium-(III)-chloride
-
 Yarrowia lipolytica NAD+ + ?
-
 ?
7.1.1.2 NADH + n-decylubiquinone + H+
-
 Yarrowia lipolytica NAD+ + n-decylubiquinol
-
 ?
7.1.1.2 NADH + O2
-
 Yarrowia lipolytica NAD+ + superoxide radical
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Yarrowia lipolytica NAD+ + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Yarrowia lipolytica
7.1.1.2 NADH:ubiquinone oxidoreductase
-
 Yarrowia lipolytica
7.1.1.2 type I NADH dehydrogenase
-
 Yarrowia lipolytica

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 NADH
-
 Yarrowia lipolytica