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Literature summary extracted from

  • Poliakov, E.; Gentleman, S.; Cunningham, F.X.; Miller-Ihli, N.J.; Redmond, T.M.
    Key role of conserved histidines in recombinant mouse beta-carotene 15,15-monooxygenase-1 activity (2005), J. Biol. Chem., 280, 29217-29223.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.63 D52A more than 50% loss of activity Mus musculus
1.13.11.63 D52A/E140A no enzymatic activity Mus musculus
1.13.11.63 E140A more than 50% loss of activity Mus musculus
1.13.11.63 E314A little reduction in enzymatic activity Mus musculus
1.13.11.63 E405A no enzymatic activity Mus musculus
1.13.11.63 E450A little reduction in enzymatic activity Mus musculus
1.13.11.63 E457A about 80% loss of activity Mus musculus
1.13.11.63 E469A about 50% decrease in activity, cells are bleached when left growing overnight Mus musculus
1.13.11.63 H172A no enzymatic activity Mus musculus
1.13.11.63 H174A little reduction in enzymatic activity Mus musculus
1.13.11.63 H237A no enzymatic activity Mus musculus
1.13.11.63 H308A no enzymatic activity Mus musculus
1.13.11.63 H309A little reduction in enzymatic activity Mus musculus
1.13.11.63 H49A little reduction in enzymatic activity Mus musculus
1.13.11.63 H514A no enzymatic activity Mus musculus
1.13.11.63 H58A little reduction in enzymatic activity Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.63 0.0009
-
 beta-carotene mutant E140A, pH 8.0, 37°C Mus musculus
1.13.11.63 0.0012
-
 beta-carotene wild-type, pH 8.0, 37°C Mus musculus
1.13.11.63 0.0023
-
 beta-carotene mutant E450A, pH 8.0, 37°C Mus musculus
1.13.11.63 0.0027
-
 beta-carotene mutant H58A, pH 8.0, 37°C Mus musculus
1.13.11.63 0.0033
-
 beta-carotene mutant E314A, pH 8.0, 37°C Mus musculus
1.13.11.63 0.0047
-
 beta-carotene mutant D52A, pH 8.0, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.63 Iron required Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Mus musculus Q9JJS6 recombinant enzyme
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.63 -20°C, 40% glycerol and presence of protease inhibitors, enzyme purified by metal affinity chromatography is stable for several weeks Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 beta-carotene + O2
-
 Mus musculus all-trans-retinal
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 Bcmo1
-
 Mus musculus