Literature summary extracted from
Okada, K.; Hase, T.
Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1 (2005), J. Biol. Chem., 280, 20672-20679.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.17.7.1 |
expression in Escherichia coli |
Thermosynechococcus vestitus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.17.7.1 |
[4Fe-4S]2+ cluster |
[4Fe-4S]2 metalloprotein |
Thermosynechococcus vestitus |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.17.7.1 |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin |
Thermosynechococcus vestitus |
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.17.7.1 |
Thermosynechococcus vestitus |
Q8DK70 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.17.7.1 |
recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein |
Thermosynechococcus vestitus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.17.7.1 |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin |
- |
Thermosynechococcus vestitus |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin |
- |
? |
|
1.17.7.1 |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin |
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis |
Thermosynechococcus vestitus |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.17.7.1 |
GcpE |
ambiguous |
Thermosynechococcus vestitus |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.17.7.1 |
metabolism |
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis |
Thermosynechococcus vestitus |