Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamaguchi, Y.; Matsumura, T.; Ichida, K.; Okamoto, K.; Nishino, T.
    Human Xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate (2007), J. Biochem., 141, 513-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.3.2 expression of wild-type and mtant enzymes in Escherichia coli strain JM109 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.4 mutant E803V, decrease in activity towards purine substrates is not due to large conformational change in the mutant protein Homo sapiens
1.17.3.2 purified recombinant mutant E308V, 15mg/ml protein in 5 mM Tris, pH 8.5, containing 1mM sodium salicylate and 0.1mM EDTA, incubation with 5 mM dithiothreitol for 1 h at room temperature, aliquots of 0.005 ml protein solution are mixed with 0.005 ml of 100 mM sodium citrate, pH 5.0, containing 8-11% polyethylene glycol 8000, 5 mM DTT, 1 mM sodium salicylate, 0.1 mM EDTA, and placed on siliconized glass plates, 7 days at 20°C, X-ray diffraction structure determination and analysis at 2.6 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.3.1 additional information mutation of two amino acid residues in the active site of human XOR (mutants E803V and R881M) for purine substrates results in conversion of the substrate preference to aldehyde oxidase type Homo sapiens
1.17.1.4 E803V almost complete loss of activity with hypoxanthine, weak activity with xanthine, significant aldehyde oxidase activity Homo sapiens
1.17.1.4 R881M almost complete loss of activity with xanthine, weak activity with hypoxanthine, significant aldehyde oxidase activity Homo sapiens
1.17.3.2 E308V site-directed mutagenesis, mutant molybdopterin domain structure, overview, the xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site, the E803V mutation almost completely abrogates the activity towards hypoxanthine as a substrate, but the decrease in activity towards purine substrate is not due to large conformational change in the mutant enzyme Homo sapiens
1.17.3.2 R881M site-directed mutagenesis, the xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site, the R881M mutant lacks activity towards xanthine, but retaines slight activity towards hypoxanthine Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 allopurinol
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.0088
-
 hypoxanthine wild-type, pH 8.5, 25°C Homo sapiens
1.17.1.4 0.0088
-
 xanthine wild-type, pH 8.5, 25°C Homo sapiens
1.17.1.4 0.072
-
 xanthine mutant E803V, pH 8.5, 25°C Homo sapiens
1.17.1.4 0.072
-
 hypoxanthine mutant R881M, pH 8.5, 25°C Homo sapiens
1.17.3.2 additional information
-
 additional information steady-state kinetics analysis of wild-type and mutant enzymes with hypoxanthine of xanthine, and of mutant enzymes with benzaldehyde or 4-(dimethyamino)cinnamaldehyde as aldehyde oxidase substrates, overview Homo sapiens
1.17.3.2 0.0017
-
 hypoxanthine pH 8.5, 25°C, recombinant wild-type enzyme Homo sapiens
1.17.3.2 0.0088
-
 xanthine pH 8.5, 25°C, recombinant wild-type enzyme Homo sapiens
1.17.3.2 0.021
-
 hypoxanthine pH 8.5, 25°C, recombinant mutant R881M Homo sapiens
1.17.3.2 0.072
-
 xanthine pH 8.5, 25°C, recombinant mutant E308V Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.3.2 Molybdenum molybdopterin domain structure of enzyme mutant E308V, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.2 hypoxanthine + 2 H2O + 2 O2 Homo sapiens
-
 urate + 2 H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 Homo sapiens roles of active site residues E308 and R881 in binding and activation of purine substrate, overview urate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Homo sapiens
-
-
-
1.17.1.4 Homo sapiens
-
-
-
1.17.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 after expression in Escherichia coli Homo sapiens
1.17.3.2 recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 by anion exchange and hydroxyapatite chromatography, ultrafiltration, and gel filtration Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.3.2 xanthine + H2O + O2 = urate + H2O2 hydrogen-bonding arrangement of the substrate-bound complex at the active site, and proposed mechanism of the purine oxidation reaction by XOR Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H+ + O2- + H2O
-
 Homo sapiens xanthine + NADH + H2O2
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Homo sapiens urate + NADH + H+
-
 ?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
 Homo sapiens urate + 2 H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Homo sapiens urate + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 roles of active site residues E308 and R881 in binding and activation of purine substrate, overview Homo sapiens urate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.3.2 xanthine oxidoreductase
-
 Homo sapiens
1.17.3.2 XOR
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.4 0.33
-
 hypoxanthine mutant R881M, pH 8.5, 25°C Homo sapiens
1.17.1.4 0.99
-
 xanthine wild-type, pH 8.5, 25°C Homo sapiens
1.17.1.4 1.35
-
 xanthine mutant E803V, pH 8.5, 25°C Homo sapiens
1.17.1.4 18.33
-
 xanthine wild-type, pH 8.5, 25°C Homo sapiens
1.17.1.4 30
-
 hypoxanthine wild-type, pH 8.5, 25°C Homo sapiens
1.17.3.2 0.33
-
 hypoxanthine pH 8.5, 25°C, recombinant mutant R881M Homo sapiens
1.17.3.2 1.35
-
 xanthine pH 8.5, 25°C, recombinant mutant E308V Homo sapiens
1.17.3.2 18.3
-
 xanthine pH 8.5, 25°C, recombinant wild-type enzyme Homo sapiens
1.17.3.2 30
-
 hypoxanthine pH 8.5, 25°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 8.5
-
 assay at Homo sapiens