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Literature summary extracted from

  • Watanabe, Y.; Makino, Y.; Omichi, K.
    Activation of 4-alpha-glucanotransferase activity of porcine liver glycogen debranching enzyme with cyclodextrins (2006), J. Biochem., 140, 135-140.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.25 cyclodextrin the extent of 4-alpha-glucanotransferase activation increases with cyclodextrin concentration before reaching a constant value Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 170000
-
gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Sus scrofa
-
-
-
3.2.1.33 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
Sus scrofa
3.2.1.33 partially purified with ammonium sulfate precipitation, DEAE-Sephacryl gel filtration, Sephacryl S-300 gel filtration, and Super Q anion exchange column high performance liquid chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.25 liver
-
Sus scrofa
-
3.2.1.33 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 1.36
-
-
Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)(Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,6))Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)Glc-pyridylamine + H2O
-
Sus scrofa maltotriose + Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)(Glcalpha(1,6))Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)Glcalpha(1,4)Glc-pyridylamine
-
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Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 dextrin 6-alpha-glucosidase
-
Sus scrofa
3.2.1.33 GDE amylo-alpha-1,6-glucosidase part of the 170000 Da multifunctional glycogen debranching enzyme (GDE) Sus scrofa