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Literature summary extracted from

  • Setoyama, C.; Nishina, Y.; Mizutani, H.; Miyahara, I.; Hirotsu, K.; Kamiya, N.; Shiga, K.; Miura, R.
    Engineering the substrate specificity of porcine kidney D-amino acid oxidase by mutagenesis of the active-site lid (2006), J. Biochem., 139, 873-879.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 E220D/Y224G kcat/KM for D-Arg is 5fold higher than wild-type value, kcat/Km for L-Lys is 2.91fold higher than wild-type value, kcat/KM for D-Ala is 124fold lower than wild-type value, kcat/Km for D-Ser is more than 110fold lower than wild-type value, kcat/Km for D-Pro is 17.5fold lower than wild-type value, kcat/Km for D-Val is 12fold lower than wild-type value, kcat/KM for D-Met is 1.5fold higher than wild-type value, kcat/KM for D-Phe is 3.3fold higher than wild-type value Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.56
-
 D-Pro wild-type enzyme Sus scrofa
1.4.3.3 0.63
-
 D-Val wild-type enzyme Sus scrofa
1.4.3.3 0.65
-
 D-Met wild-type enzyme Sus scrofa
1.4.3.3 0.67
-
 D-Met mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 0.67
-
 D-Met mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 0.77
-
 D-Ala wild-type enzyme Sus scrofa
1.4.3.3 0.77
-
 D-Phe mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 1.4
-
 D-Phe wild-type enzyme Sus scrofa
1.4.3.3 1.4
-
 D-Phe mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 3
-
 D-Arg mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 3
-
 D-Arg mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 3.1
-
 D-Val mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 3.3
-
 D-Ser wild-type enzyme Sus scrofa
1.4.3.3 3.7
-
 D-Val mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 4.1
-
 D-Pro mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 7.7
-
 D-Pro mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 10
-
 D-Lys wild-type enzyme Sus scrofa
1.4.3.3 13
-
 D-Arg wild-type enzyme Sus scrofa
1.4.3.3 14
-
 D-Lys mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 20
-
 D-Lys mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 44
-
 D-Ala mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 46
-
 D-Ala mutant enzyme R221D/Y224G Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-Ala + H2O + O2
-
 Sus scrofa pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Arg + H2O + O2
-
 Sus scrofa 5-guanidino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-Lys + H2O + O2
-
 Sus scrofa 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-Met + H2O + O2
-
 Sus scrofa 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Phe + H2O + O2
-
 Sus scrofa phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Pro + H2O + O2
-
 Sus scrofa 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2
-
 Sus scrofa 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 D-Val + H2O + O2
-
 Sus scrofa alpha-ketoisovalerate + NH3 + H2O2
-
 ?
1.4.3.3 additional information activity with D-Asp is not detectable Sus scrofa ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 0.052 2.1 D-Pro wild-type enzyme Sus scrofa
1.4.3.3 0.25
-
 D-Val mutant enzyme G222D/Y224G Sus scrofa
1.4.3.3 0.4
-
 D-Phe mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 0.433
-
 D-Val mutant enzymeY224G Sus scrofa
1.4.3.3 0.75
-
 D-Ala mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 0.75
-
 D-Val mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 0.8
-
 D-Lys wild-type enzyme Sus scrofa
1.4.3.3 1.05
-
 D-Val mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 1.33
-
 D-Met mutant enzyme I223D/Y224G Sus scrofa
1.4.3.3 1.38
-
 D-Met mutant enzymeY224G Sus scrofa
1.4.3.3 1.67
-
 D-Pro mutant enzymeY224G Sus scrofa
1.4.3.3 1.83
-
 D-Arg mutant enzyme I223D/Y224G Sus scrofa
1.4.3.3 2
-
 D-Arg mutant enzymeY224G Sus scrofa
1.4.3.3 2.2
-
 D-Ser mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 2.33
-
 D-Lys mutant enzymeY224G Sus scrofa
1.4.3.3 2.5
-
 D-Val wild-type enzyme Sus scrofa
1.4.3.3 2.5
-
 D-Met mutant enzyme G222D/Y224G Sus scrofa
1.4.3.3 3
-
 D-Ser wild-type enzyme Sus scrofa
1.4.3.3 3
-
 D-Ala mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 3
-
 D-Phe mutant enzyme I223D/Y224G Sus scrofa
1.4.3.3 3
-
 D-Lys mutant enzyme I223D/Y224G Sus scrofa
1.4.3.3 3.17
-
 D-Phe mutant enzymeY224G Sus scrofa
1.4.3.3 3.33
-
 D-Lys mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 3.5
-
 D-Arg wild-type enzyme Sus scrofa
1.4.3.3 3.5
-
 D-Phe mutant enzyme G222D/Y224G Sus scrofa
1.4.3.3 3.5
-
 D-Pro mutant enzyme G222D/Y224G Sus scrofa
1.4.3.3 3.83
-
 D-Pro mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 4
-
 D-Arg mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 4
-
 D-Arg mutant enzyme G222D/Y224G Sus scrofa
1.4.3.3 4.33
-
 D-Pro mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 5
-
 D-Lys mutant enzyme G222D/Y224G Sus scrofa
1.4.3.3 5
-
 D-Lys mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 6.5
-
 D-Ala wild-type enzyme Sus scrofa
1.4.3.3 6.83
-
 D-Met wild-type enzyme Sus scrofa
1.4.3.3 7.5
-
 D-Arg mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 9
-
 D-Met mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 10.33
-
 D-Pro wild-type enzyme Sus scrofa
1.4.3.3 10.5
-
 D-Met mutant enzyme E220D/Y224G Sus scrofa
1.4.3.3 13
-
 D-Phe mutant enzyme R221D/Y224G Sus scrofa
1.4.3.3 16.7
-
 D-Phe wild-type enzyme Sus scrofa
1.4.3.3 53.33
-
 D-Phe mutant enzyme E220D/Y224G Sus scrofa