Literature summary extracted from
Abe, K.; Kaya, S.; Taniguchi, K.; Hayashi, Y.; Imagawa, T.; Kikumoto, M.; Oiwa, K.; Sakaguchi, K.
Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase (2005), J. Biochem., 138, 293-301.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
7.2.2.19 |
additional information |
no protective effect during C12E8-solubilization of gastric H/K-ATPase by choline |
Sus scrofa |
|
Application
EC Number |
Application |
Comment |
Organism |
---|
7.2.2.19 |
additional information |
strong evidence that the catalytic unit of C12E8-solubilized gastric H/K-ATPase is a tetraprotomer |
Sus scrofa |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
7.2.2.19 |
Fluorescein 5'-isothiocyanate |
fluorescein 5'-isothiocyanate modified enzyme has 0.5-1.5% residual ATPase activity compared to the unmodified enzyme |
Sus scrofa |
|
7.2.2.19 |
SCH28080 |
- |
Sus scrofa |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
7.2.2.19 |
membrane |
- |
Sus scrofa |
16020 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
7.2.2.19 |
K+ |
stabilizes both oligomeric assembly and enzyme activity in C12E8-solubilized gastric H/K-ATPase, 20-40 mM protects |
Sus scrofa |
|
7.2.2.19 |
additional information |
no protective effect during C12E8-solubilization of gastric H/K-ATPase by Na+ and Li+ |
Sus scrofa |
|
7.2.2.19 |
Rb+ |
protects gastric H/K-ATPase during C12E8-solubilization |
Sus scrofa |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
7.2.2.19 |
Sus scrofa |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
7.2.2.19 |
by SDS-PAGE |
Sus scrofa |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
7.2.2.19 |
6.3 |
- |
purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C |
Sus scrofa |
7.2.2.19 |
7.5 |
- |
purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C |
Sus scrofa |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
7.2.2.19 |
-80°C, EGTA/Tris buffer, pH 7.4 |
Sus scrofa |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
7.2.2.19 |
gastric H/K-ATPase |
- |
Sus scrofa |