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Literature summary extracted from

  • Llacer, J.L.; Polo, L.M.; Tavarez, S.; Alarcon, B.; Hilario, R.; Rubio, V.
    The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction (2007), J. Bacteriol., 189, 1254-1265.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.2.2 additional information putative gene for carbamate kinase is ef0735 on the DNA strand of the Enterococcus faecalis V583 chromosome. The arginine deiminase pathway of arginine catabolism probably supplies the genes for PTC and CK but not those for the agmatine/putrescine antiporter Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.6 expressed in Escherichia coli Enterococcus faecalis
2.1.3.6 gene ef0732, gene cluster organization, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli Enterococcus faecalis
3.5.3.12 expressed in Escherichia coli BL21 (DE3) cells Enterococcus faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.6 purified recombinant enzyme in presence of inhibitor N-(phosphonoacetyl)-putrescine, X-ray diffraction structure determination and analysis at 3.0 A resolution Enterococcus faecalis
2.1.3.6 using the sparse-matrix sampling vapor-diffusion method. The addition of N-(phosphonoacetyl)-putrescine to the crystallization drop strongly improves the results of the crystallization Enterococcus faecalis
3.5.3.12 hanging drop vapour diffusion method with in 50 mM Tris-HCl, pH 7.45, 1 mM dithiothreitol and 20 mM NaCl Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.6 N-(phosphonoacetyl)-putrescine i.e. PAPU, highly potent and selective inhibitor Enterococcus faecalis
2.1.3.6 phosphonoacetyl-L-aspartate
-
 Enterococcus faecalis
2.1.3.6 phosphonoacetyl-L-ornithine
-
 Enterococcus faecalis
3.5.3.12 arcaine competitive inhibitor Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.6 0.0023
-
 putrescine
-
 Enterococcus faecalis
2.1.3.6 0.058
-
 Carbamoyl phosphate
-
 Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.6 40000
-
 gel filtration Enterococcus faecalis
3.5.3.12 46000
-
 SDS-PAGE Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.6 carbamoyl phosphate + L-ornithine Enterococcus faecalis low activity, reaction of EC 2.1.3.3 phosphate + N-carbamoyl-L-ornithine
-
 ?
2.1.3.6 carbamoyl phosphate + L-ornithine Enterococcus faecalis ATCC 700802 low activity, reaction of EC 2.1.3.3 phosphate + N-carbamoyl-L-ornithine
-
 ?
2.1.3.6 carbamoyl phosphate + putrescine Enterococcus faecalis
-
 phosphate + N-carbamoyl-putrescine
-
 ?
2.1.3.6 carbamoyl phosphate + putrescine Enterococcus faecalis ATCC 700802
-
 phosphate + N-carbamoyl-putrescine
-
 ?
2.1.3.6 additional information Enterococcus faecalis the enzyme catalyzes the second of three steps of ATP synthesis from agmatine ?
-
 ?
2.1.3.6 additional information Enterococcus faecalis ATCC 700802 the enzyme catalyzes the second of three steps of ATP synthesis from agmatine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.6 Enterococcus faecalis
-
-
-
2.1.3.6 Enterococcus faecalis
-
 strain SD10, gene ef0732
-
2.1.3.6 Enterococcus faecalis ATCC 700802
-
 strain SD10, gene ef0732
-
2.7.2.2 Enterococcus faecalis
-
 SD10 strain
-
2.7.2.2 Enterococcus faecalis ATCC 700802
-
 SD10 strain
-
3.5.3.12 Enterococcus faecalis Q837U5 strain SD10
-
3.5.3.12 Enterococcus faecalis ATCC 700802 Q837U5 strain SD10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.6 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Enterococcus faecalis
3.5.3.12 HisTrap Ni-affinity column chromatography and Superdex 200 HR gel filtration Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.6 460
-
 non-recombinant enzyme from Enterococcus faecalis Enterococcus faecalis
2.1.3.6 597
-
 recombinant enzyme from Enterococcus faecalis Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.6 carbamoyl phosphate + cadaverine only weak activity with cadaverine a substrate Enterococcus faecalis phosphate + N-carbamoylcadaverine
-
 ?
2.1.3.6 carbamoyl phosphate + cadaverine only weak activity with cadaverine a substrate Enterococcus faecalis ATCC 700802 phosphate + N-carbamoylcadaverine
-
 ?
2.1.3.6 carbamoyl phosphate + L-ornithine low activity, reaction of EC 2.1.3.3 Enterococcus faecalis phosphate + N-carbamoyl-L-ornithine
-
 ?
2.1.3.6 carbamoyl phosphate + L-ornithine low activity, reaction of EC 2.1.3.3 Enterococcus faecalis ATCC 700802 phosphate + N-carbamoyl-L-ornithine
-
 ?
2.1.3.6 carbamoyl phosphate + ornithine only weak activity with ornithine as a substrate Enterococcus faecalis phosphate + N-carbamoylornithine
-
 ?
2.1.3.6 carbamoyl phosphate + putrescine
-
 Enterococcus faecalis phosphate + N-carbamoylputrescine
-
 ?
2.1.3.6 carbamoyl phosphate + putrescine
-
 Enterococcus faecalis ATCC 700802 phosphate + N-carbamoylputrescine
-
 ?
2.1.3.6 carbamoyl phosphate + putrescine
-
 Enterococcus faecalis phosphate + N-carbamoyl-putrescine
-
 ?
2.1.3.6 carbamoyl phosphate + putrescine
-
 Enterococcus faecalis ATCC 700802 phosphate + N-carbamoyl-putrescine
-
 ?
2.1.3.6 additional information the enzyme catalyzes the second of three steps of ATP synthesis from agmatine Enterococcus faecalis ?
-
 ?
2.1.3.6 additional information the enzyme catalyzes the second of three steps of ATP synthesis from agmatine Enterococcus faecalis ATCC 700802 ?
-
 ?
3.5.3.12 agmatine + H2O highly specific for agmatine Enterococcus faecalis N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O highly specific for agmatine Enterococcus faecalis ATCC 700802 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 additional information does not use L-arginine, L-argininamide, or arcaine (1,4-diguanidinobutane) as substrates Enterococcus faecalis ?
-
 ?
3.5.3.12 additional information does not use L-arginine, L-argininamide, or arcaine (1,4-diguanidinobutane) as substrates Enterococcus faecalis ATCC 700802 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.6 trimer
-
 Enterococcus faecalis
2.1.3.6 trimer crystal strucutre Enterococcus faecalis
3.5.3.12 tetramer X-ray crystallography and gel filtration Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
2.1.3.6 PTC
-
 Enterococcus faecalis
2.1.3.6 putrescine transcarbamylase
-
 Enterococcus faecalis
3.5.3.12 AgDI
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.6 37
-
 assay at Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.6 7
-
 assay at Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.6 0.00001
-
 N-(phosphonoacetyl)-putrescine recombinant enzyme Enterococcus faecalis
2.1.3.6 10
-
 N-(phosphonoacetyl)-putrescine
-
 Enterococcus faecalis
3.5.3.12 0.028
-
 arcaine at 37°C Enterococcus faecalis