EC Number | Cloned (Comment) | Organism |
---|---|---|
7.3.2.6 | expression in Escherichia coli BL21(DE3) | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.6 | additional information | - |
additional information | the extremely high affinity of the enzyme for tungstate results in a very steep binding curve | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.3.2.6 | 40000 | - |
native PAGE | Pyrococcus furiosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.6 | ATP + H2O + tungstate/out | Pyrococcus furiosus | the very high affinity WtpABC transporter explains the ability of Pyrococcus furiosus cells to scavenge traces of tungstate from growth medium | ADP + phosphate + tungstate/in | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.3.2.6 | Pyrococcus furiosus | Q8U4K5 | tungstate transport protein A, part of the ABC transporter system | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.3.2.6 | - |
Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.6 | ATP + H2O + tungstate/out | the very high affinity WtpABC transporter explains the ability of Pyrococcus furiosus cells to scavenge traces of tungstate from growth medium | Pyrococcus furiosus | ADP + phosphate + tungstate/in | - |
? | |
7.3.2.6 | ATP + H2O + tungstate/out | although reversible binding of molybdate or tungstate by the soluble component WtpA of the archaeal WtpABC transporter enforces a quasi-octahedral coordination onto the molybdenum or tungsten, this does not induce any redox chemistry of the +VI state | Pyrococcus furiosus | ADP + phosphate + tungstate/in | - |
? | |
7.3.2.6 | ATP + H2O + tungstate/out | when the protein is incubated with a mixture of tungstate and molybdate, the amount of bound tungstate is significantly higher, even in the presence of an excess of molybdate during the incubation. WtpA is able to bind tungstate and molybdate, with a preference for tungstate, without changing the oligomeric state of the protein | Pyrococcus furiosus | ADP + phosphate + tungstate/in | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.3.2.6 | monomer | - |
Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.3.2.6 | PF0080 | - |
Pyrococcus furiosus |
7.3.2.6 | tungstate-specific ABC transporter | - |
Pyrococcus furiosus |
7.3.2.6 | WptA | tungstate transport protein A, part of the ABC transporter system | Pyrococcus furiosus |
7.3.2.6 | WtpABC | - |
Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.3.2.6 | 80 | - |
6 h, no precipiotation is observed | Pyrococcus furiosus |