EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.3.20 | heterologously expressed in Escherichia coli | Chloroflexus aurantiacus |
4.1.3.25 | expressed in Escherichia coli BL21 cells | Chloroflexus aurantiacus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.3.20 | 0.08 | - |
succinyl-CoA | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus | |
2.8.3.20 | 2.2 | - |
(R)-citramalate | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.3.20 | additional information | activity is not dependent on Mg2+ | Chloroflexus aurantiacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.3.20 | 44000 | - |
2 * 44000, SDS-PAGE | Chloroflexus aurantiacus |
2.8.3.20 | 90000 | - |
gel filtration | Chloroflexus aurantiacus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.20 | succinyl-CoA + (R)-citramalate | Chloroflexus aurantiacus | the enzyme catalyzes a steps in the glyoxylate assimilation route | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | Chloroflexus aurantiacus DSM 636 | the enzyme catalyzes a steps in the glyoxylate assimilation route | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | the enzyme catalyzes a steps in the glyoxylate assimilation route | succinate + (R)-citramalyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.20 | Chloroflexus aurantiacus | - |
- |
- |
2.8.3.20 | Chloroflexus aurantiacus DSM 636 | - |
- |
- |
4.1.3.46 | Chloroflexus aurantiacus | - |
- |
- |
4.1.3.46 | Chloroflexus aurantiacus OK-70-fl / DSM 636 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.3.20 | partial | Chloroflexus aurantiacus |
4.1.3.46 | ammonium sulfate precipitation, gel filtration, and Mono Q chromatography | Chloroflexus aurantiacus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.8.3.20 | succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA | (1) | Chloroflexus aurantiacus | |
2.8.3.20 | succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA | (2) | Chloroflexus aurantiacus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.3.20 | 17.5 | - |
55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus |
4.1.3.46 | 1.5 | - |
recombinant enzyme | Chloroflexus aurantiacus |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.3.20 | 4°C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity | Chloroflexus aurantiacus |
2.8.3.20 | frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity | Chloroflexus aurantiacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.20 | succinyl-CoA + (R)-citramalate | - |
Chloroflexus aurantiacus | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | - |
Chloroflexus aurantiacus DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-citramalate | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-malate | 88% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus | succinate + (R)-malyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-malate | 88% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus DSM 636 | succinate + (R)-malyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + (R)-malate | 88% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + (R)-malyl-CoA | - |
? | |
2.8.3.20 | succinyl-CoA + itaconate | 80% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus | succinate + ? | - |
? | |
2.8.3.20 | succinyl-CoA + itaconate | 80% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus DSM 636 | succinate + ? | - |
? | |
2.8.3.20 | succinyl-CoA + itaconate | 80% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + ? | - |
? | |
4.1.3.46 | (3R)-citramalyl-CoA | - |
Chloroflexus aurantiacus | acetyl-CoA + pyruvate | - |
? | |
4.1.3.46 | (3R)-citramalyl-CoA | - |
Chloroflexus aurantiacus DSM 636 | acetyl-CoA + pyruvate | - |
? | |
4.1.3.46 | (3R)-citramalyl-CoA | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | acetyl-CoA + pyruvate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.3.20 | homodimer | 2 * 44000, SDS-PAGE | Chloroflexus aurantiacus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.3.20 | SCT | - |
Chloroflexus aurantiacus |
4.1.3.25 | D-citramalyl-CoA lyase | - |
Chloroflexus aurantiacus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.20 | 55 | - |
- |
Chloroflexus aurantiacus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.20 | 55 | - |
stable | Chloroflexus aurantiacus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.3.20 | 13 | - |
succinyl-CoA | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus | |
2.8.3.20 | 13 | - |
(R)-citramalate | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.20 | 6.5 | - |
at 55°C | Chloroflexus aurantiacus |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.8.3.20 | Chloroflexus aurantiacus | upregulated under autotrophic conditions | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.8.3.20 | physiological function | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus |