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Literature summary extracted from

  • Patridge, E.V.; Ferry, J.G.
    WrbA from Escherichia coli and Archaeoglobus fulgidus is an NAD(P)H:quinone oxidoreductase (2006), J. Bacteriol., 188, 3498-3506.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.2 additional information enzyme knockout mutant, no phenotypes, but N-trichloromethyl-mercapto-4-cyclohexen-1,2-dicarboximide and 8-hydroxyquinoline significantly inhibit growth of mutant relative to growth of wild-type Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0058
-
 1,4-benzoquinone pH 7.2, 37°C Escherichia coli
1.6.5.2 0.014
-
 NADH pH 7.2, 37°C Escherichia coli
1.6.5.2 0.019
-
 NADH pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 0.031
-
 NADPH pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 0.037
-
 1,4-benzoquinone pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 0.17
-
 NADPH pH 7.2, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 21200
-
 4 * 21200, SDS-PAGE Escherichia coli
1.6.5.2 22100
-
 2 * 22100, SDS-PAGE Archaeoglobus fulgidus
1.6.5.2 22100
-
 4 * 22100, SDS-PAGE Archaeoglobus fulgidus
1.6.5.2 52000
-
 gel filtration Archaeoglobus fulgidus
1.6.5.2 79000
-
 dynamic light scattering Escherichia coli
1.6.5.2 89000
-
 dynamic light scattering Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.2 additional information Escherichia coli role of enzyme in protecting against environmental stressors ?
-
 ?
1.6.5.2 additional information Archaeoglobus fulgidus role of enzyme in protecting against environmental stressors ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Archaeoglobus fulgidus P58795 tryptophan repressor-binding protein
-
1.6.5.2 Escherichia coli P0A8G6 tryptophan repressor-binding protein
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 50
-
 substrate [Fe(CN)6]3-, pH 7.2, 37°C Escherichia coli
1.6.5.2 160
-
 substrate 2,6-dichlorophenolindophenol, pH 7.2, 37°C Escherichia coli
1.6.5.2 430
-
 substrate menadione, pH 7.2, 37°C Escherichia coli
1.6.5.2 560
-
 substrate 1,4-naphthoquinone, pH 7.2, 37°C Escherichia coli
1.6.5.2 930
-
 substrate 2,3-dihydroxy-5-methyl-1,4-benzoquinone, pH 7.2, 37°C Escherichia coli
1.6.5.2 990
-
 substrate 1,4-benzoquinone, pH 7.2, 37°C Escherichia coli
1.6.5.2 1300
-
 substrate menadione, pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 1400
-
 substrate 2,6-dichlorophenolindophenol, pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 1400
-
 substrate [Fe(CN)6]3-, pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 6700
-
 substrate 2,3-dihydroxy-5-methyl-1,4-benzoquinone, pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 8900
-
 substrate 1,4-naphthoquinone, pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 25000
-
 substrate 1,4-benzoquinone, pH 7.2, 37°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 1,4-benzoquinone + NAD(P)H + H+
-
 Escherichia coli 1,4-benzoquinol + NAD(P)+
-
 ?
1.6.5.2 1,4-benzoquinone + NAD(P)H + H+
-
 Archaeoglobus fulgidus 1,4-benzoquinol + NAD(P)+
-
 ?
1.6.5.2 1,4-benzoquinone + NADH + H+
-
 Escherichia coli 1,4-benzoquinol + NAD+
-
 r
1.6.5.2 1,4-benzoquinone + NADH + H+
-
 Archaeoglobus fulgidus 1,4-benzoquinol + NAD+
-
 r
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Escherichia coli 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Archaeoglobus fulgidus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 2,3-dihydroxy-5-methyl-1,4-benzoquinone + NAD(P)H
-
 Escherichia coli 2,3-dihydroxy-5-methyl-1,4-benzoquinol + NAD(P)+
-
 ?
1.6.5.2 2,3-dihydroxy-5-methyl-1,4-benzoquinone + NAD(P)H
-
 Archaeoglobus fulgidus 2,3-dihydroxy-5-methyl-1,4-benzoquinol + NAD(P)+
-
 ?
1.6.5.2 menadione + NAD(P)H
-
 Escherichia coli reduced menadione + NAD(P)+
-
 ?
1.6.5.2 menadione + NAD(P)H
-
 Archaeoglobus fulgidus reduced menadione + NAD(P)+
-
 ?
1.6.5.2 additional information role of enzyme in protecting against environmental stressors Escherichia coli ?
-
 ?
1.6.5.2 additional information role of enzyme in protecting against environmental stressors Archaeoglobus fulgidus ?
-
 ?
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Escherichia coli NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Archaeoglobus fulgidus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.5.2 [Fe(CN)6]3- + NAD(P)H
-
 Escherichia coli [Fe(CN)6]4- + NAD(P)+
-
 ?
1.6.5.2 [Fe(CN)6]3- + NAD(P)H
-
 Archaeoglobus fulgidus [Fe(CN)6]4- + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 dimer 2 * 22100, SDS-PAGE Archaeoglobus fulgidus
1.6.5.2 tetramer 4 * 21200, SDS-PAGE Escherichia coli
1.6.5.2 tetramer 4 * 22100, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 WrbA
-
 Escherichia coli
1.6.5.2 WrbA
-
 Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 6
-
 NADPH pH 7.2, 37°C Escherichia coli
1.6.5.2 8.9
-
 NADH pH 7.2, 37°C Escherichia coli
1.6.5.2 120
-
 NADH pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 170
-
 NADPH pH 7.2, 37°C Archaeoglobus fulgidus
1.6.5.2 370
-
 1,4-benzoquinone pH 7.2, 37°C Escherichia coli
1.6.5.2 3200
-
 1,4-benzoquinone pH 7.2, 37°C Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.2 5 8.5 more than 95% of maximum activity within this range Archaeoglobus fulgidus
1.6.5.2 6 8 more than 95% of maximum activity within this range Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FMN one molecule per monomer Escherichia coli
1.6.5.2 FMN one molecule per monomer Archaeoglobus fulgidus
1.6.5.2 NADH
-
 Escherichia coli
1.6.5.2 NADPH
-
 Escherichia coli