EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.267 | gene ispC, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), Rv2870c complements an inactivated chromosomal copy of IspC in Salmonella enterica, and the complemented strain is sensitive to fosmidomycin | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate | substrate inhibition | Mycobacterium tuberculosis | |
1.1.1.267 | EDTA | complete inhibition at 5 mM | Mycobacterium tuberculosis | |
1.1.1.267 | fosmidomycin | IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteria and other organisms | Mycobacterium tuberculosis | |
1.1.1.267 | additional information | no substrate inhibition by 2-C-methyl-D-erythritol 4-phosphate, NADPH or NADP+ | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.267 | 0.0297 | - |
NADPH | pH 7.9, 30°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
1.1.1.267 | 0.0471 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.9, 30°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
1.1.1.267 | 0.174 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.9, 30°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
1.1.1.267 | 0.561 | - |
NADP+ | pH 7.9, 30°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.267 | cytosol | - |
Mycobacterium tuberculosis | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | Mg2+ | absolutely dependent on, optimal at 4 mM | Mycobacterium tuberculosis | |
1.1.1.267 | additional information | Mn2+, Ca2+, Co2+, Zn2+, and Fe2+ ions are unable to support activity | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.267 | 45000 | - |
x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis | first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis H37Rv | first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.267 | Mycobacterium tuberculosis | - |
gene ispC | - |
1.1.1.267 | Mycobacterium tuberculosis H37Rv | - |
gene ispC | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.267 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview | Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | the forward direction is preferred | Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview | Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | the forward direction is preferred | Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.267 | ? | x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.267 | IspC | - |
Mycobacterium tuberculosis |
1.1.1.267 | Rv2870c | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.267 | 30 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.267 | 7.5 | 7.9 | forward and reverse reaction | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.267 | 5 | 9 | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | NADP+ | - |
Mycobacterium tuberculosis | |
1.1.1.267 | NADPH | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.267 | 1.1 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.9, 30°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.267 | 0.00031 | - |
IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteri | Mycobacterium tuberculosis | fosmidomycin | |
1.1.1.267 | 0.0027 | - |
IC50: 310 nM for the forward reaction, 0.0027 mM for the reverse reaction, Gram positive bacteria, including Mycobacterium tuberculosis, are resistant against the antibiotic fosmidomycin, which is an inhibitor of the enzyme from most gram-negative bacteri | Mycobacterium tuberculosis | fosmidomycin |