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Literature summary extracted from

  • Abe, T.; Masai, E.; Miyauchi, K.; Katayama, Y.; Fukuda, M.
    A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6 (2005), J. Bacteriol., 187, 2030-2037.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.341 tetrahydrofolate dependent on Sphingomonas paucimobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.341 expression in Escherichia coli Sphingomonas paucimobilis
2.1.1.341 gene ligM, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strains JM109 and BL21(DE3) Sphingomonas paucimobilis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.341 additional information disruption of ligM leads to significant growth retardation on both vanillate and syringate, a ligM desA double mutant completely looses the ability to transform vanillate, phenotypes, overview Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.341 vanillate + tetrahydrofolate Sphingomonas paucimobilis
-
 protocatechuate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 vanillate + tetrahydrofolate Sphingomonas paucimobilis
-
 protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?
2.1.1.341 vanillate + tetrahydrofolate Sphingomonas paucimobilis SYK-6
-
 protocatechuate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 vanillate + tetrahydrofolate Sphingomonas paucimobilis SYK-6
-
 protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.341 Sphingomonas paucimobilis Q60FX1
-
-
2.1.1.341 Sphingomonas paucimobilis Q60FX1 gene ligM
-
2.1.1.341 Sphingomonas paucimobilis SYK-6 Q60FX1
-
-
2.1.1.341 Sphingomonas paucimobilis SYK-6 Q60FX1 gene ligM
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.341 3-O-methylgallate + tetrahydrofolate
-
 Sphingomonas paucimobilis gallate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 additional information syringate is no substrate Sphingomonas paucimobilis ?
-
 ?
2.1.1.341 additional information no substrate: syringate Sphingomonas paucimobilis ?
-
 ?
2.1.1.341 additional information syringate is no substrate Sphingomonas paucimobilis SYK-6 ?
-
 ?
2.1.1.341 additional information no substrate: syringate Sphingomonas paucimobilis SYK-6 ?
-
 ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate
-
 ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate ?
2.1.1.341 vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.341 LigM
-
 Sphingomonas paucimobilis
2.1.1.341 tetrahydrofolate-dependent O-demethylase
-
 Sphingomonas paucimobilis
2.1.1.341 vanillate/3-O-methylgallate O-demethylase
-
 Sphingomonas paucimobilis
2.1.1.341 vanillate/3MGA O-demethylase
-
 Sphingomonas paucimobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.341 30
-
 assay at Sphingomonas paucimobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.341 8
-
 assay at Sphingomonas paucimobilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.341 NADH
-
 Sphingomonas paucimobilis

General Information

EC Number General Information Comment Organism
2.1.1.341 physiological function disruption of LigM leads to significant growth retardation on both vanillate and syringate. The ability of the mutant to transform vanillate is markedly decreased, and it completely lost the 3-O-methylgallate O-demethylase activity. A LigM syringate demethylase DesA double mutant completely lost the ability to transform vanillate Sphingomonas paucimobilis