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Literature summary extracted from

  • Singh, V.; Schramm, V.L.
    Transition-state structure of human 5-methylthioadenosine phosphorylase (2006), J. Am. Chem. Soc., 128, 14691-14696.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 expression of the N-terminally His6-tagged enzyme in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 additional information
-
 additional information kinetic isotope effects measured on the arsenolysis of 5'-methylthioadenosine catalyzed by the enzyme, the enzyme has a dissociative SN1 transition state with its cationic center at the anomeric carbon and insignificant bond order to the leaving group, the transition state has cationic character at the anomeric carbon and is anionic at the 3'-OH oxygen with an anionic leaving group, late SN1 transition state with significant participation of the phosphate nucleophile, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.28 S-methyl-5'-thioadenosine + phosphate Homo sapiens
-
 adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate transition state structure, computational modeling of the transition state, overview Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 S-methyl-5'-thioadenosine + arsenate arsenolysis Homo sapiens adenine + S-methyl-5-thio-alpha-D-ribose 1-arsenate
-
 ?
2.4.2.28 S-methyl-5'-thioadenosine + phosphate
-
 Homo sapiens adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 S-methyl-5'-thioadenosine + phosphate phosphorolysis Homo sapiens adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 5-methylthioadenosine phosphorylase
-
 Homo sapiens
2.4.2.28 MTAP
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 7.5
-
 assay at Homo sapiens