EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.25 | additional information | - |
additional information | analysis of kinetics of single turnover reactions of the two intermediates with ethyl vinyl ether and diethyl ether, single- and double-mixing stopped-flow optical spectroscopy, overview, stopped-flow kinetics, analytical model of transient kinetics | Methylococcus capsulatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.13.25 | soluble | - |
Methylococcus capsulatus | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NADH + O2 | Methylococcus capsulatus | - |
methanol + NAD+ + H2O | - |
? | |
1.14.13.25 | methane + NADH + O2 | Methylococcus capsulatus Bath | - |
methanol + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.25 | Methylococcus capsulatus | - |
- |
- |
1.14.13.25 | Methylococcus capsulatus Bath | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.25 | native MMOH, in the purification procedure includes anion exchange chromatography and gel filtration | Methylococcus capsulatus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.13.25 | methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | the soluble enzyme utilizes a carboxylate-bridged diiron center and dioxygen, a di(my-oxo)diiron(IV) intermediate termed Q is responsible for the catalytic activity with hydrocarbons, the peroxodiiron(III) intermediate precedes Q formation in the catalytic cycle | Methylococcus capsulatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 0.02 | 0.04 | purified MMOH, substrate propylene | Methylococcus capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NADH + O2 | - |
Methylococcus capsulatus | methanol + NAD+ + H2O | - |
? | |
1.14.13.25 | methane + NADH + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD+ + H2O | - |
? | |
1.14.13.25 | propylene + NADH + O2 | the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene | Methylococcus capsulatus | propylene oxide + NAD+ + H2O | - |
? | |
1.14.13.25 | propylene + NADH + O2 | the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene | Methylococcus capsulatus Bath | propylene oxide + NAD+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.25 | MMOH | - |
Methylococcus capsulatus |
1.14.13.25 | soluble methane monooxygenase hydroxylase | - |
Methylococcus capsulatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 45 | - |
assay at | Methylococcus capsulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 7 | - |
assay at | Methylococcus capsulatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | NADH | - |
Methylococcus capsulatus |