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Literature summary extracted from

  • Garbe, L.A.; Barbosa de Almeida, R.; Nagel, R.; Wackerbauer, K.; Tressl, R.
    Dual positional and stereospecificity of lipoxygenase isoenzymes from germinating barley (green malt): biotransformation of free and esterified linoleic acid (2006), J. Agric. Food Chem., 54, 946-955.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Hordeum vulgare
-
-
-
1.13.11.58 Hordeum vulgare P29114
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.12
-
 Hordeum vulgare
1.13.11.58
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 linoleate + O2
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 Hordeum vulgare (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate trilinolein and extracted polar and nonpolar lipids are oxidized with a ratio (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate to (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate of 6:94, 2:98, and 7:93. The stereoisomer ratios (S:R) of (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate with these substrates are 82:18, 92:8, and 91:9 whereas (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is analyzed (R)-configured with 41:59, 47:53, and 40:60 (S:R) ?
1.13.11.58 linoleate + O2 low catalytic activity with complex substrates as compared to free linoleic acid. Residual relative activities lower than 1% with the substrates dilinolein, trilinolein, and 1-palmitoyl-2-linoleoyl-glycero-3-phosphocholine and with extracted lipids from malt confirm this supposition. However, LOX1 catalyzes HPODE formation from PamLinGroPCho with high regioselectivity (9-hydroperoxy-(10E,12Z)-octadecadienoate:13-hydroperoxy-(10E,12Z)-octadecadienoate) and high (9S)-hydroperoxy-(10E,12Z)-octadecadienoate stereoselectivity (S:R) (92:8) Hordeum vulgare (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 LOX2
-
 Hordeum vulgare
1.13.11.58 LOX1
-
 Hordeum vulgare