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Literature summary extracted from

  • Kim, B.G.; Jung, B.R.; Lee, Y.; Hur, H.G.; Lim, Y.; Ahn, J.H.
    Regiospecific flavonoid 7-O-methylation with Streptomyces avermitilis O-methyltransferase expressed in Escherichia coli (2006), J. Agric. Food Chem., 54, 823-828.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.B75 expressed in Escherichia coli as a His-tag fusion protein Streptomyces avermitilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.B75 38000
-
 x * 38000, SDS-PAGE Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.B75 Streptomyces avermitilis
-
-
-
2.1.1.B75 Streptomyces avermitilis MA-4680
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.B75
-
 Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.B75 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin Streptomyces avermitilis S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin ?
2.1.1.B75 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin ?
2.1.1.B75 S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin, 119% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin, 119% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, active is identical to that with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin, 57% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin, 57% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin, 23.4% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?
2.1.1.B75 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin, 23.4% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?
2.1.1.B75 S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein. 59% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. 126% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.B75 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. 126% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone) Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Subunits

EC Number Subunits Comment Organism
2.1.1.B75 ? x * 38000, SDS-PAGE Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.B75 SaOMT-2
-
 Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.B75 30
-
 assay at Streptomyces avermitilis