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Literature summary extracted from

  • Spagna, G.; Barbagallo, R.N.; Chisari, M.; Branca, F.
    Characterization of a tomato polyphenol oxidase and its role in browning and lycopene content (2005), J. Agric. Food Chem., 53, 2032-2038.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 tropolone
-
Solanum lycopersicum
1.14.18.1 tropolone
-
Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.1 additional information Solanum lycopersicum PPO activity is associated with color changes associated with browning and lycopene degradation, the commercial variety Naomi is more susceptible to enzymatic browning than the local varieties Pizzutello, Rosa Maletto and PO228, due to higher PPO activity levels, lycopene is an antioxidant agent that reconstitutes the polyphenols oxidized by the action of PPO ?
-
?
1.14.18.1 additional information Solanum lycopersicum PPO activity is associated with color changes associated with browning and lycopene degradation, the commercial variety Naomi is more susceptible to enzymatic browning than the local varieties Pizzutello, Rosa Maletto and PO228, due to higher PPO activity levels, lycopene is an antioxidant agent that reconstitutes the polyphenols oxidized by the action of PPO ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Solanum lycopersicum
-
var. Naomi, Pizzutello, Rosa Maletto, and PO228, physicochemical properties of the different tomato varieties, overview
-
1.14.18.1 Solanum lycopersicum
-
var. Naomi, Pizzutello, Rosa Maletto, and PO228, physicochemical properties of the different tomato varieties, overview
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit
-
Solanum lycopersicum
-
1.14.18.1 fruit
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
analysis of browning parameters Solanum lycopersicum
1.14.18.1 additional information
-
analysis of browning parameters Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
1.10.3.1 4°C, 55% of the maximum activity at this cold-storage temperature Solanum lycopersicum
1.14.18.1 4°C, 55% of the maximum activity at this cold-storage temperature Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 4-methylcatechol + O2
-
Solanum lycopersicum 4-methyl-1,2-benzoquinone + H2O
-
?
1.10.3.1 catechol + 1/2 O2
-
Solanum lycopersicum 1,2-benzoquinone + H2O
-
?
1.10.3.1 additional information PPO activity is associated with color changes associated with browning and lycopene degradation, the commercial variety Naomi is more susceptible to enzymatic browning than the local varieties Pizzutello, Rosa Maletto and PO228, due to higher PPO activity levels, lycopene is an antioxidant agent that reconstitutes the polyphenols oxidized by the action of PPO Solanum lycopersicum ?
-
?
1.14.18.1 3,4-dihydroxyphenylacetic acid + O2
-
Solanum lycopersicum ?
-
?
1.14.18.1 3,4-dihydroxyphenylacetic acid + O2 3-methylbenzothyazolinone hydrazone, MBTH, as chromophore coupling agent Solanum lycopersicum ?
-
?
1.14.18.1 dopamine + O2
-
Solanum lycopersicum ?
-
?
1.14.18.1 L-DOPA + O2
-
Solanum lycopersicum ?
-
?
1.14.18.1 additional information PPO activity is associated with color changes associated with browning and lycopene degradation, the commercial variety Naomi is more susceptible to enzymatic browning than the local varieties Pizzutello, Rosa Maletto and PO228, due to higher PPO activity levels, lycopene is an antioxidant agent that reconstitutes the polyphenols oxidized by the action of PPO Solanum lycopersicum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 More cf. EC 1.14.18.1 Solanum lycopersicum
1.10.3.1 polyphenol oxidase
-
Solanum lycopersicum
1.10.3.1 PPO
-
Solanum lycopersicum
1.14.18.1 More cf. EC 1.10.3.1 Solanum lycopersicum
1.14.18.1 polyphenol oxidase
-
Solanum lycopersicum
1.14.18.1 PPO
-
Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 40
-
-
Solanum lycopersicum
1.14.18.1 40
-
-
Solanum lycopersicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 30 50
-
Solanum lycopersicum
1.14.18.1 30 50
-
Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 4.8
-
-
Solanum lycopersicum
1.14.18.1 4.8
-
-
Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 4.1 4.4 74% and 87% of maximal activity Solanum lycopersicum
1.14.18.1 4.1 4.4 74% and 87% of maximal activity Solanum lycopersicum