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Literature summary extracted from

  • Herve, C.; Fondrevez, M.; Cheron, A.; Barloy-Hubler, F.; Jan, G.
    Transcarboxylase mRNA: a marker which evidences P. freudenreichii survival and metabolic activity during its transit in the human gut (2007), Int. J. Food Microbiol., 113, 303-314.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.3.1 analysis use of enzyme as a molecular marker for specific detection of Propionibacterium freudenreichii in human microbiota. Rapid quantification of bacteria by real time PCR using enzyme operon Propionibacterium freudenreichii
2.1.3.1 diagnostics monitoring propionibacteria population and propionic fermentation in human trials constitutes a crucial step in determining Propionibacterium freudenreichii ability to prevent intestinal disorders Propionibacterium freudenreichii
2.1.3.1 medicine use of enzyme as a molecular marker for specific detection of Propionibacterium freudenreichii in human microbiota. Rapid quantification of bacteria by real time PCR using enzyme operon. Propionibacterium freudenreichii not only survives but remains metabolically active in the human gut Propionibacterium freudenreichii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.1 12000
-
 determined by 2D gel electrophoresis, 1.3S subunit Propionibacterium freudenreichii
2.1.3.1 12370
-
 calculated molecular mass, 1.3S subunit Propionibacterium freudenreichii
2.1.3.1 55620
-
 calculated molecular mass, 5S subunit Propionibacterium freudenreichii
2.1.3.1 56400
-
 calculated molecular mass, 12S subunit Propionibacterium freudenreichii
2.1.3.1 63000
-
 determined by 2D gel electrophoresis, 5S subunit Propionibacterium freudenreichii
2.1.3.1 67000
-
 determined by 2D gel electrophoresis, 12S subunit Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.1 (S)-methylmalonyl-CoA + pyruvate Propionibacterium freudenreichii
-
 propanoyl-CoA + oxaloacetate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.1 Propionibacterium freudenreichii
-
-
-
2.1.3.1 Propionibacterium freudenreichii
-
 enzyme is expressed during propionic fermentation, ceases in starved cells and is not affected by digestive stress
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.1 radioactive labeling of Propionibacterium freudenreichii proteins is performed, analysis of the proteins by two-dimensional gel electrophoresis follows Propionibacterium freudenreichii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.1 (S)-methylmalonyl-CoA + pyruvate
-
 Propionibacterium freudenreichii propanoyl-CoA + oxaloacetate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.3.1 multimer
-
 Propionibacterium freudenreichii

Synonyms

EC Number Synonyms Comment Organism
2.1.3.1 methyl malonyl CoA carboxyl transferase
-
 Propionibacterium freudenreichii
2.1.3.1 transcarboxylase
-
 Propionibacterium freudenreichii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.3.1 Propionibacterium freudenreichii determined by 2D gel electrophoresis, 1.3S subunit
-
 4.9
2.1.3.1 Propionibacterium freudenreichii determined by 2D gel electrophoresis, 5S subunit
-
 5.12
2.1.3.1 Propionibacterium freudenreichii determined by 2D gel electrophoresis, 12S subunit
-
 5.15
2.1.3.1 Propionibacterium freudenreichii calculated pI-value, 1.3S subunit
-
 5.18
2.1.3.1 Propionibacterium freudenreichii calculated pI-value, 12S subunit
-
 5.26
2.1.3.1 Propionibacterium freudenreichii calculated pI-value, 5S subunit
-
 5.27