EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.3.2 | DNA and amino acid sequence determination and analysis | Cyathus bulleri |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | 2-mercaptoethanol | competitive, complete inhibition | Cyathus bulleri | |
1.10.3.2 | cetyltrimethyl ammonium bromide | 50% inhibition | Cyathus bulleri | |
1.10.3.2 | HgCl2 | competitive, complete inhibition | Cyathus bulleri | |
1.10.3.2 | KCN | competitive, complete inhibition | Cyathus bulleri | |
1.10.3.2 | NaN3 | competitive, complete inhibition | Cyathus bulleri | |
1.10.3.2 | SDS | competitive, complete inhibition | Cyathus bulleri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 14 | - |
ferulic acid | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 22 | - |
guaiacol | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 48.6 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 56 | - |
2,6-dimethoxyphenol | pH 5.2, 45°C | Cyathus bulleri |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Cu2+ | the gene sequence encodes copper-binding domains I and II | Cyathus bulleri | |
1.10.3.2 | additional information | no effect by EDTA | Cyathus bulleri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 60000 | - |
gel filtration | Cyathus bulleri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Cyathus bulleri | Q8X1D1 | birds nest fungus | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | native enzyme 95.7fold to homogeneity by anion exchange chromatography and gel filtration | Cyathus bulleri |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.10.3.2 | mycelium | - |
Cyathus bulleri | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 226 | - |
purified enzyme | Cyathus bulleri |
EC Number | Storage Stability | Organism |
---|---|---|
1.10.3.2 | 4°C, purified enzyme, 50 mM citrate phosphate, pH 5.0, no loss in activity after 1 month | Cyathus bulleri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Cyathus bulleri | ? | - |
? | |
1.10.3.2 | 2,6-dimethoxyphenol + O2 | - |
Cyathus bulleri | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
1.10.3.2 | 3,4-dihydroxybenzoic acid + O2 | - |
Cyathus bulleri | ? | - |
? | |
1.10.3.2 | ferulic acid + O2 | - |
Cyathus bulleri | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | - |
Cyathus bulleri | ? | - |
? | |
1.10.3.2 | additional information | substrate specificity, overview, no activity with tyrosine, phenol, 2,4-dichlorophenol, and 5-sulphosalycylic acid | Cyathus bulleri | ? | - |
? | |
1.10.3.2 | syringaldehyde + O2 | - |
Cyathus bulleri | ? | - |
? | |
1.10.3.2 | vanillin + O2 | - |
Cyathus bulleri | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.2 | monomer | 1 * 60000, SDS-PAGE | Cyathus bulleri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 45 | - |
- |
Cyathus bulleri |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 25 | 70 | stable at, inactivation above | Cyathus bulleri |
1.10.3.2 | 80 | - |
complete inactivation | Cyathus bulleri |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 5.2 | - |
ferulic acid | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 5.5 | - |
guaiacol | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 95.6 | - |
guaiacol | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 180 | - |
2,6-dimethoxyphenol | pH 5.2, 45°C | Cyathus bulleri | |
1.10.3.2 | 204 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.2, 45°C | Cyathus bulleri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 5.2 | - |
- |
Cyathus bulleri |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 2 | 6 | sharp decline in activity above pH 6.0 | Cyathus bulleri |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.10.3.2 | Cyathus bulleri | isoelectric focusing | - |
3.7 |