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Literature summary extracted from

  • Hayeshi, R.; Mutingwende, I.; Mavengere, W.; Masiyanise, V.; Mukanganyama, S.
    The inhibition of human glutathione S-transferases activity by plant polyphenolic compounds ellagic acid and curcumin (2007), Food Chem. Toxicol., 45, 286-295.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expression of isozymes in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 (+)-catechin hydrate inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
2.5.1.18 curcumin inhibition of isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1 Homo sapiens
2.5.1.18 daidzein inhibition of isozymes GST A1-1, M1-1, and P1-1 Homo sapiens
2.5.1.18 ellagic acid inhibition of isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1, effects on substrate kinetics, overview Homo sapiens
2.5.1.18 ferulic acid inhibition of isozyme GST M1-1 Homo sapiens
2.5.1.18 genistein inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
2.5.1.18 kaempferol inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
2.5.1.18 quercetin inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
2.5.1.18 resveratrol inhibition of isozymes GST A2-2, M1-1, M2-2, and P1-1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.72
-
 1-chloro-2,4-dinitrobenzene pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens
2.5.1.18 0.86
-
 glutathione pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens
-
 GST isozymes A1-1, A2-2, M1-1, M2-2, and P1-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant isozymes from Escherichia coli by S-hexylglutathione affinity chromatography to homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 78
-
 purified recombinant isozyme GST A2-2 Homo sapiens
2.5.1.18 84
-
 purified recombinant isozyme GST A1-1 Homo sapiens
2.5.1.18 129
-
 purified recombinant isozyme GST P1-1 Homo sapiens
2.5.1.18 157
-
 purified recombinant isozyme GST M1-1 Homo sapiens
2.5.1.18 182
-
 purified recombinant isozyme GST M2-2 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens chloride + 2,4-dinitrophenyl-glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Homo sapiens
2.5.1.18 GST
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 30
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 47.3
-
 glutathione pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens
2.5.1.18 82
-
 1-chloro-2,4-dinitrobenzene pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens
2.5.1.18 103.3
-
 1-chloro-2,4-dinitrobenzene pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.4
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 additional information
-
-
 Homo sapiens additional information
2.5.1.18 0.00004 0.005 pH 7.4, 30°C, purified recombinant isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1 Homo sapiens curcumin
2.5.1.18 0.00013 0.005 pH 7.4, 30°C, purified recombinant isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1 Homo sapiens ellagic acid
2.5.1.18 0.0003 0.0005 pH 7.4, 30°C, purified recombinant isozymes GST M1-1 and M2-2 Homo sapiens quercetin
2.5.1.18 0.001 0.002 pH 7.4, 30°C, purified recombinant isozymes GST M1-1 and M2-2 Homo sapiens kaempferol
2.5.1.18 0.004 0.005 pH 7.4, 30°C, purified recombinant isozymes GST M1-1 and M2-2 Homo sapiens genistein