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Literature summary extracted from

  • Kawaguchi, K.; Shinoda, Y.; Yurimoto, H.; Sakai, Y.; Kato, N.
    Purification and characterization of benzoate-CoA ligase from Magnetospirillum sp. strain TS-6 capable of aerobic and anaerobic degradation of aromatic compounds (2006), FEMS Microbiol. Lett., 257, 208-213.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.03
-
 benzoate apparent value, at pH 9.0 Magnetospirillum sp.
6.2.1.25 0.092
-
 CoA apparent value, at pH 9.0 Magnetospirillum sp.
6.2.1.25 0.265
-
 ATP apparent value, at pH 9.0 Magnetospirillum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 63000
-
 2 * 63000, gel filtration Magnetospirillum sp.
6.2.1.25 120000
-
 SDS-PAGE Magnetospirillum sp.
6.2.1.25 126000
-
 gel filtration Magnetospirillum sp.

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Magnetospirillum sp. Q25C16 strainTS-6
-
6.2.1.25 Magnetospirillum sp. TS-6 Q25C16 strainTS-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25 DEAE-Toyopearl column chromatography, Phenyl-Sepharose Fast Flow column chromatography, and Superose 12 gel filtration Magnetospirillum sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 0.081
-
 soluble protein, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10mM KCl, 2.5mM MgCl2, 10mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2U of lactate dehydrogenase, and 0.1 mM substrate Magnetospirillum sp.
6.2.1.25 13.4
-
 after 166fold purification, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10 mM KCl, 2.5 mM MgCl2, 10 mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2 mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2 units of lactate dehydrogenase, and 0.1 mM substrate Magnetospirillum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-aminobenzoate + CoA 6.7% and 10% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 61% and 57% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 61% and 57% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate Magnetospirillum sp. TS-6 AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-aminobenzoate + CoA 3.7% activity in anaerobically grown cells, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + 3-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 16% activity in aerobically and anaerobically grown cells, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 16% activity in aerobically and anaerobically grown cells, compared to the activity with benzoate Magnetospirillum sp. TS-6 AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-aminobenzoate + CoA 5.6% activity in anaerobically grown cells, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + 4-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA 72% and 69% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA 72% and 69% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate Magnetospirillum sp. TS-6 AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA 100% activity in aerobically and anaerobically grown cells Magnetospirillum sp. AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA 100% activity in aerobically and anaerobically grown cells Magnetospirillum sp. TS-6 AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + picolinate + CoA 3.4% and 2.7% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate Magnetospirillum sp. AMP + diphosphate + picolinyl-CoA
-
 ?
6.2.1.25 additional information little or no activity with 2-hydroxybenzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-chlorobenzoate, 3-chlorobenzoate, 4-chlorobenzoate, cyclohexane carboxylate, and nicotinate Magnetospirillum sp. ?
-
 ?
6.2.1.25 additional information little or no activity with 2-hydroxybenzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-chlorobenzoate, 3-chlorobenzoate, 4-chlorobenzoate, cyclohexane carboxylate, and nicotinate Magnetospirillum sp. TS-6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 homodimer 2 * 63000, gel filtration Magnetospirillum sp.

Synonyms

EC Number Synonyms Comment Organism
6.2.1.25 Bcl
-
 Magnetospirillum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.25 9
-
-
 Magnetospirillum sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.25 7 9.5
-
 Magnetospirillum sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.25 ATP
-
 Magnetospirillum sp.