EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.1 | expressed in Escherichia coli | Pyrococcus furiosus |
2.4.2.1 | expression in Escherichia coli | Pyrococcus furiosus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.1 | C254S/C256S | reduced thermodynamic and kinetic stability of the mutant with respect to the wild-type PfPNP | Pyrococcus furiosus |
2.4.2.1 | C254S/C256S | Tm-value is 8°C lower than Tm-value of wild-type enzyme | Pyrococcus furiosus |
EC Number | General Stability | Organism |
---|---|---|
2.4.2.1 | limited proteolysis indicated that the only proteolytic cleavage site is localized in the C-terminal region | Pyrococcus furiosus |
2.4.2.1 | phosphate is able to increase the already high stability of PfPNP toward SDS | Pyrococcus furiosus |
2.4.2.1 | remarkable SDS-resistance, enzyme remains fully active after 30 min in 2% SDS at 50°C and retains 60% residual activity after 5 min at 90°C | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | 0.122 | - |
guanosine | 80°C | Pyrococcus furiosus | |
2.4.2.1 | 0.322 | - |
Inosine | 80°C | Pyrococcus furiosus | |
2.4.2.1 | 0.322 | - |
Inosine | 80°C, pH 7.4 | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 28977 | - |
6 * 28977, calculated from sequence | Pyrococcus furiosus |
2.4.2.1 | 29000 | - |
6 * 29000, SDS-PAGE | Pyrococcus furiosus |
2.4.2.1 | 29208 | - |
6 * 29208, calculated from sequence | Pyrococcus furiosus |
2.4.2.1 | 180000 | - |
gel filtration | Pyrococcus furiosus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.4.2.1 | SDS | 2%, the enzyme remains fully active after 30 min incubation at 50°C and still retains 60% residual activity after 5 min incubation at 90°C. Phosphate is able to increase the already high stability of PfPNP toward the detergent | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.1 | Pyrococcus furiosus | - |
- |
- |
2.4.2.1 | Pyrococcus furiosus | Q8U2I1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.1 | - |
Pyrococcus furiosus |
2.4.2.1 | recombinant | Pyrococcus furiosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 0.0179 | - |
- |
Pyrococcus furiosus |
2.4.2.1 | 0.0179 | - |
80°C, production of hypoxanthine | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.1 | guanosine + phosphate | - |
Pyrococcus furiosus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | guanosine + phosphate | absolute specificity for inosine and guanosine | Pyrococcus furiosus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | inosine + phosphate | - |
Pyrococcus furiosus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | inosine + phosphate | absolute specificity for inosine and guanosine | Pyrococcus furiosus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.1 | hexamer | 6 * 28977, calculated from sequence | Pyrococcus furiosus |
2.4.2.1 | hexamer | 6 * 29000, SDS-PAGE | Pyrococcus furiosus |
2.4.2.1 | hexamer | 6 * 29208, calculated from sequence | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.1 | PfPNP | - |
Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 120 | - |
- |
Pyrococcus furiosus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 75 | 133 | 75°C: about 55% of maximal activity, 133°C: about 50% of maximal activity | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | additional information | - |
three pairs of intrasubunit disulfide bridges play a role in the stability of the enzyme against thermal inactivation | Pyrococcus furiosus |
2.4.2.1 | 100 | - |
4 h, stable | Pyrococcus furiosus |
2.4.2.1 | 100 | - |
4 h, stable, wild-type enzyme. Mutant enzyme C254S/C256S shows 38% residual activity after 4 h incubation | Pyrococcus furiosus |
2.4.2.1 | 102 | - |
Tm-value for mutant enzyme C254S/C256S | Pyrococcus furiosus |
2.4.2.1 | 105 | - |
half-life: 69 min | Pyrococcus furiosus |
2.4.2.1 | 105 | - |
half-life: 69 min for wild-type enzyme, 35.5 min for mutant enzyme C254S/C256S | Pyrococcus furiosus |
2.4.2.1 | 110 | - |
Tm-value is 110°C, half-life: 12 min | Pyrococcus furiosus |
2.4.2.1 | 110 | - |
half-life: 12 min, Tm-value 110°C increases to 120°C in the presence of 100 mM phosphate, wild-type enzyme | Pyrococcus furiosus |
2.4.2.1 | 115 | - |
half-life: 5 min | Pyrococcus furiosus |
2.4.2.1 | 115 | - |
half-life: 5 min, wild-type enzyme | Pyrococcus furiosus |
2.4.2.1 | 120 | - |
Tm-value in presence of 100 mM phosphate | Pyrococcus furiosus |
2.4.2.1 | 120 | - |
Tm-value 110°C increases to 120°C in the presence of 100 mM phosphate, wild-type enzyme | Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | 28.05 | - |
guanosine | 80°C | Pyrococcus furiosus | |
2.4.2.1 | 84.19 | - |
Inosine | 80°C | Pyrococcus furiosus |