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Literature summary extracted from

  • Wanibuchi, K.; Zhang, P.; Abe, T.; Morita, H.; Kohno, T.; Chen, G.; Noguchi, H.; Abe, I.
    An acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata (2007), FEBS J., 274, 1073-1082.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.159 expressed in Escherichia coli with an additional hexahistidine tag at the N-terminus Huperzia serrata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.159 19.7
-
 4-coumaroyl-CoA pH 7.0, 30°C Huperzia serrata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.159 45831
-
 x * 45831, calculated Huperzia serrata

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.159 Huperzia serrata A3E7Z7 recombinant enzyme expressed in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.159 4-coumaroyl-CoA + 3 malonyl-CoA
-
 Huperzia serrata naringenin chalcone + 4 CoA + 3 CO2 small amounts of bisnoryangonin and 4-coumaroyltriacetic acid lactone are formed as byproducts ?
2.3.1.159 4-methoxycinnamoyl-CoA + malonyl-CoA
-
 Huperzia serrata ? + CoA + CO2
-
 ?
2.3.1.159 acetyl-CoA + malonyl-CoA
-
 Huperzia serrata ? + CoA + CO2
-
 ?
2.3.1.159 benzoyl-CoA + malonyl-CoA
-
 Huperzia serrata 2,3',4,6-tetrahydroxybenzophenone + CoA + CO2
-
 ?
2.3.1.159 hexanoyl-CoA + malonyl-CoA
-
 Huperzia serrata ? + CoA + CO2
-
 ?
2.3.1.159 additional information enzyme accepts bulky substrates as starters and carries out one to three condensations to yield various aromatic tetraketides along with some di- and triketides Huperzia serrata ?
-
 ?
2.3.1.159 N-methylanthraniloyl-CoA + 3 malonyl-CoA
-
 Huperzia serrata 1,3-dihydroxy-N-methylacridone + 4 CoA + 3 CO2
-
 ?
2.3.1.159 phenylacetyl-CoA + malonyl-CoA
-
 Huperzia serrata 2,4,6-trihydroxyphenylbenzylketone + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.159 ? x * 45831, calculated Huperzia serrata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.159 0.034
-
 4-coumaroyl-CoA pH 7.0, 30°C Huperzia serrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.159 7 8
-
 Huperzia serrata