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Literature summary extracted from

  • Mann, S.; Ploux, O.
    7,8-Diaminoperlargonic acid aminotransferase from Mycobacterium tuberculosis, a potential therapeutic target. Characterization and inhibition studies (2006), FEBS J., 273, 4778-4789.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.62 overproduced in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 3-(cis-4-aminocyclohexa-2,5-dien-1-yl)propan-1-ol suicide substrate, compound at 0.1 mg/l completely inhibits the growth of an Escherichia coli C268bioA mutant strain transformed with a plasmid expressing the Myobaterium tuberculosis bioA gene, coding for diaminopelargonic acid aminotransferase; suicide substrate, irreversible inhibition Mycobacterium tuberculosis
2.6.1.62 8-Amino-7-oxononanoate substrate inhibition Mycobacterium tuberculosis
2.6.1.62 amiclenomycin suicide substrate; suicide substrate, irreversible inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 0.0038
-
 8-Amino-7-oxononanoate
-
 Mycobacterium tuberculosis
2.6.1.62 0.0038
-
 8-Amino-7-oxononanoate pH 8.6, 37°C Mycobacterium tuberculosis
2.6.1.62 0.78
-
 S-adenosyl-L-methionine
-
 Mycobacterium tuberculosis
2.6.1.62 0.78
-
 S-adenosyl-L-methionine pH 8.6, 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.62 45000
-
 4 * 45000, SDS-PAGE Mycobacterium tuberculosis
2.6.1.62 189000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Mycobacterium tuberculosis
-
-
-
2.6.1.62 Mycobacterium tuberculosis P9WQ81
-
-
2.6.1.62 Mycobacterium tuberculosis ATCC 25618 P9WQ81
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.62
-
 Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate enzyme shows a Ping Pong Bi Bi kinetic mechanism with strong substrate inhibition Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate ping pong bi bi kinetic mechanism with strong substrate inhibition Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis ATCC 25618 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.62 tetramer 4 * 45000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.62 7,8-diaminoperlargonic acid aminotransferase
-
 Mycobacterium tuberculosis
2.6.1.62 DAPA AT
-
 Mycobacterium tuberculosis
2.6.1.62 diaminoperlargonic acid aminotransferase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.62 0.017
-
 S-adenosyl-L-methionine pH 8.6, 37°C Mycobacterium tuberculosis
2.6.1.62 1
-
 8-Amino-7-oxononanoate
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.62 pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.62 0.012
-
 amiclenomycin
-
 Mycobacterium tuberculosis
2.6.1.62 0.012
-
 amiclenomycin pH 8.6, 37°C Mycobacterium tuberculosis
2.6.1.62 0.014
-
 8-Amino-7-oxononanoate
-
 Mycobacterium tuberculosis
2.6.1.62 0.014
-
 3-(cis-4-aminocyclohexa-2,5-dien-1-yl)propan-1-ol pH 8.6, 37°C Mycobacterium tuberculosis
2.6.1.62 0.02
-
 3-(cis-4-aminocyclohexa-2,5-dien-1-yl)propan-1-ol
-
 Mycobacterium tuberculosis
2.6.1.62 0.02
-
 3-(cis-4-aminocyclohexa-2,5-dien-1-yl)propan-1-ol pH 8.6, 37°C Mycobacterium tuberculosis