Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liepins, J.; Kuorelahti, S.; Penttilae, M.; Richard, P.
    Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina (2006), FEBS J., 273, 4229-4235.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.156 expressed in Saccharomyces cerevisiae Trichoderma reesei
1.1.1.372 expressed in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.156 0.05
-
 NADPH pH 7.0, 30°C Trichoderma reesei
1.1.1.156 0.11
-
 NADP+ pH 9.5, 30°C Trichoderma reesei
1.1.1.156 1
-
 dihydroxyacetone pH 7.0, 30°C Trichoderma reesei
1.1.1.156 350
-
 glycerol pH 9.5, 30°C Trichoderma reesei
1.1.1.372 0.4
-
 methylglyoxal at pH 7.0 and 30°C Trichoderma reesei
1.1.1.372 0.9
-
 D-glyceraldehyde at pH 7.0 and 30°C Trichoderma reesei
1.1.1.372 0.9
-
 diacetyl at pH 7.0 and 30°C Trichoderma reesei
1.1.1.372 0.9
-
 L-glyceraldehyde at pH 7.0 and 30°C Trichoderma reesei
1.1.1.372 2.4
-
 Glyoxal at pH 7.0 and 30°C Trichoderma reesei
1.1.1.372 5.8
-
 dihydroxyacetone at pH 7.0 and 30°C Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.156 35660
-
-
 Trichoderma reesei
1.1.1.372 36232
-
 x * 36232, calculated from amino acid sequence Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.372 D-glyceraldehyde + NADPH + H+ Trichoderma reesei
-
 glycerol + NADP+
-
 ?
1.1.1.372 D-glyceraldehyde + NADPH + H+ Trichoderma reesei RUT C-30
-
 glycerol + NADP+
-
 ?
1.1.1.372 L-glyceraldehyde + NADPH + H+ Trichoderma reesei the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde glycerol + NADP+
-
 ?
1.1.1.372 L-glyceraldehyde + NADPH + H+ Trichoderma reesei RUT C-30 the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde glycerol + NADP+
-
 ?
1.1.1.372 additional information Trichoderma reesei no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) ?
-
 ?
1.1.1.372 additional information Trichoderma reesei RUT C-30 no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.156 Trichoderma reesei Q0GYU4 also known as hypocrea jecorina
-
1.1.1.372 Trichoderma reesei Q0GYU5 synonym Trichoderma reesei
-
1.1.1.372 Trichoderma reesei RUT C-30 Q0GYU5 synonym Trichoderma reesei
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.372 Ni-NTA column chromatography Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.156 dihydroxyacetone + NADPH + H+
-
 Trichoderma reesei glycerol + NADP
-
 ir
1.1.1.372 D-glyceraldehyde + NADPH + H+
-
 Trichoderma reesei glycerol + NADP+
-
 ?
1.1.1.372 D-glyceraldehyde + NADPH + H+
-
 Trichoderma reesei RUT C-30 glycerol + NADP+
-
 ?
1.1.1.372 diacetyl + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
1.1.1.372 dihydroxyacetone + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
1.1.1.372 glyoxal + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
1.1.1.372 glyoxal + NADPH + H+
-
 Trichoderma reesei RUT C-30 ?
-
 ?
1.1.1.372 L-glyceraldehyde + NADPH + H+ the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde Trichoderma reesei glycerol + NADP+
-
 ?
1.1.1.372 L-glyceraldehyde + NADPH + H+ the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde Trichoderma reesei RUT C-30 glycerol + NADP+
-
 ?
1.1.1.372 methylglyoxal + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
1.1.1.372 methylglyoxal + NADPH + H+
-
 Trichoderma reesei RUT C-30 ?
-
 ?
1.1.1.372 additional information no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) Trichoderma reesei ?
-
 ?
1.1.1.372 additional information no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose) Trichoderma reesei RUT C-30 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.372 ? x * 36232, calculated from amino acid sequence Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.156 GLD2
-
 Trichoderma reesei
1.1.1.372 gld1
-
 Trichoderma reesei
1.1.1.372 NADP-dependent glycerol dehydrogenase
-
 Trichoderma reesei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.156 NADPH
-
 Trichoderma reesei
1.1.1.372 NADPH specific cofactor Trichoderma reesei

General Information

EC Number General Information Comment Organism
1.1.1.372 metabolism the enzyme is active in pathways for sugar acid catabolism such as D-galacturonate catabolism Trichoderma reesei